year 15, Issue 1 (January - February 2021)
Iran J Med Microbiol 2021, 15(1): 46-66 |
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Fatemeh Habibinava1 
, Mohammad Reza Zolfaghari 2, Salehe Sabouri Shahrbabak3 , Mohsen Zargar1 , Mohammad Soleimani4
, Mohammad Reza Zolfaghari 2, Salehe Sabouri Shahrbabak3 , Mohsen Zargar1 , Mohammad Soleimani4
1- Department of Microbiology, School of Basic Sciences, Qom Branch, Islamic Azad University, Qom, Iran
2- Department of Microbiology, School of Basic Sciences, Qom Branch, Islamic Azad University, Qom, Iran , mreza.zolfaghary@gmail.com
3- Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
4- Department of Microbiology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
2- Department of Microbiology, School of Basic Sciences, Qom Branch, Islamic Azad University, Qom, Iran , mreza.zolfaghary@gmail.com
3- Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
4- Department of Microbiology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
Abstract: (5390 Views)
Background: This study aimed to isolate phages able to lyse some strains of multiple drug-resistant (MDR)-Klebsiella pneumoniae (named vB_Kp1 and vB_Kp2) and Enterobacter aerogenes (named vB_Ea1) from swage.
Materials and Methods: Different K. pneumoniae and E. aerogenes strains were isolated from clinical specimens during January-September 2018 in three hospitals of Amol, Mazandaran, Iran. Kirby-Bauer disc diffusion method was used to determine the resistance profiles of these isolates using different antibiotics. The MDR strains were selected for investigating the effect of isolated phages from wastewater and hospital sewage. The presence of phage was confirmed by plaque formation. The isolated bacteriophages were enriched, concentrated, and stained. Afterwards, a transmitting electron microscope (TEM) was applied to observe the morphology of the bacteriophages. Phage identification tests, including host range and one-step growth, were performed.
Results: The TEM analysis revealed that three phages had an icosahedral capsid and long contractile tail being classified as a member of the Myoviridae family. Phages were able to lyse 14 (56%) of the 25 MDR isolated bacterial strains. The one-step growth curve showed large bursts and short latent times.
Conclusions: The formation of clear plaques demonstrates the high lyse power of phages. Therefore, they have good potential for further analysis for clinical use as a therapeutic agent in the future.
Keywords: Enterobacter aerogenes, Klebsiella pneumoniae, Multiple drug-resistant infections, Phage therapy
Type of Study: Original Research Article |
Subject:
Medical Virology
Received: 2020/09/16 | Accepted: 2020/11/2 | ePublished: 2021/01/10
Received: 2020/09/16 | Accepted: 2020/11/2 | ePublished: 2021/01/10
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