year 1, Issue 4 (Winter 2008)                   Iran J Med Microbiol 2008, 1(4): 51-47 | Back to browse issues page

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Khaleghi M, Kasra Kermanshahi R, Zarkesh Esfahani S H. Assessment of the S-layer nanostructure production under anaerobic and 5% CO2 conditions in Lactobacillus acidophilus ATCC4356. Iran J Med Microbiol 2008; 1 (4) :51-47
1- Department of biology, Faculty of sciences, Isfahan University, Isfahan
Abstract:   (26648 Views)
Background and Objectives: Surface layers (S-layers) are monomolecular crystalline arrays composed of protein or glycoprotein subunits. S-layers have been identified as the outermost structure of cell envelope in numerous organisms from the domains Bacteria and Archaea. The S-layer protein subunits are noncovalently linked to each other as well as to the supporting cell wall, and can be disintegrated into monomers by denaturing agents such as urea or guanidine hydrochloride. The attention to S-layers is due to their structural properties and the capability of self-assembly on various surfaces. These properties are the new fields of researches in the nanobiotechnology, nanotechnology, biotechnology and biomedical sciences. Material and Methods: Lactobacillus acidophilus ATCC4356 was cultivated in MRS broth under anaerobic and 5% CO2 conditions at 37°C. Surface proteins of L. acidophilus were extracted by treatment of whole cells with (4M) guanidine hydrochloride. Then these extracts were analyzed by SDS-PAGE. Results: When the S-layer proteins were extracted and analyzed by SDS-PAGE, they were shown by coomassi brilliant blue staining. The S-proteins , bands of 43 kDa were visible and compared together.
Conclusion: It has been shown that the anaerobic condition is better than 5% CO2 condition for extraction Slayer protein in Lactobacillus acidophilus ATCC4356.
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Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2007/12/2 | Accepted: 2013/11/14 | ePublished: 2013/11/14

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