TY - JOUR JF - Iran-J-Med-Microbiol JO - Iran J Med Microbiol VL - 4 IS - 3 PY - 2010 Y1 - 2010/12/01 TI - HBV genotype and subtypes in HBV infected patients with and without HIV infection TT - فراوانی ژنوتایپ و ساب‌ تایپ‌های HBV در بیماران مبتلا به عفونت همزمانHIV/HBV و HBV به‌تنهایی N2 - Background and Objective: The infections caused by hepatitis B virus (HBV) and HIV are a part of world health complications. Chronic hepatitis B affects nearly 10% of HIV-infected patients. Four million people are estimated to have HBV/HIV co-infection worldwide. The aim of the study was to determine the prevalence of HBV genotypes and subtypes in Iranian HBV infected patients with or without HIV coinfection.Material and Methods: Sera from 58 Hepatitis B patients including 30(51.7%) with HBV infection and 28 (48.3%) with HIV/HBV co-infection were collected from medical centers in Tehran, Fars and Hormozgan provinces in years 2007-2009. DNA was extracted and surface gene (S region) was amplified by Nested PCR and subjected to sequencing. Phylogenic analysis was done using Mega4 software and drawing phylogenetic tree. Results: Genotype D was the only genotype detected in both groups of patients. HBV subtypes observed in patients without HIV coinfection were ayw2 (27.90, 30%), ayw3(1.30, 3/3%) and ayw4 (2.6, 30.7%) while the subtypes found in HBV patients coinfected with HIV were included ayw2 (10.28, 7.35%), ayw3 (11.28, 39.3%) and ayw4 (7.28, 25%). The findings revealed a significant correlation between the types of infection (HBV/HIV co-infection or HBV mono infection) and the subtypes (P SP - 29 EP - 35 AU - Farahnifar, Ahmad AU - Aghasadeghi, Mohammadreza AU - Bahramali, Golnaz AU - Sadat, Seyed Mehdi AU - Siadat, Seyed Davar AU - Moharez, Minoo AU - Froughi, Maryam AU - Javadi, Foziye AU - Behbahani, Abbas AU - Rouhvand, Farzin AD - ) Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran,Iran. KW - HBV/HIV KW - HBV KW - Genotype KW - Subtype KW - Iran UR - http://ijmm.ir/article-1-33-en.html ER -