year 13, Issue 1 (March - April 2019)
Iran J Med Microbiol 2019, 13(1): 56-68 |
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Arian S, Kaboosi H, Heshmatipour Z, Khazaei -Koohpar Z, Peyravii-Ghadikolaii F. Molecular analysis of seven Lactobacillus strains isolated from human origin in West of Mazandaran.. Iran J Med Microbiol 2019; 13 (1) :56-68
URL: http://ijmm.ir/article-1-919-en.html
URL: http://ijmm.ir/article-1-919-en.html
Simin Arian1 
, Hami Kaboosi 2, Zaheir Heshmatipour3 , Zeinab Khazaei -Koohpar4 , Fatemeh Peyravii-Ghadikolaii5
, Hami Kaboosi 2, Zaheir Heshmatipour3 , Zeinab Khazaei -Koohpar4 , Fatemeh Peyravii-Ghadikolaii5
1- Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran , hkaboosi@gmail.com
3- Department of Microbiology, Faculty of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
4- Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
5- Department of Biology, Ghaemshahr Branch, Islamic Azad University, Ghaemshahr, Iran
2- Department of Microbiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran , hkaboosi@gmail.com
3- Department of Microbiology, Faculty of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
4- Department of Cellular and Molecular Biology, Faculty of Biological Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
5- Department of Biology, Ghaemshahr Branch, Islamic Azad University, Ghaemshahr, Iran
Abstract: (5708 Views)
Background and Aims: Colorectal cancer is considered to be an important cause of morbidity and mortality worldwide. Anti-cancer properties of probiotic lactic acid bacteria are considerable for treatment of cancers such as colorectal cancer. The aim of this study was to investigate the molecular characterization of probiotic bacterial isolates from collected stool specimens in west of Mazandaran province, in order to confirm the probiotic effect of isolated strains, so to compare their effect on cancer cell lineages, in future studies.
Materials and Methods: In this study, out of 60 stool samples collected from healthy children and adult people, 50 bacterial strains were isolated. Antibiotic susceptibility was performed by 15 antibiotics for 7 strains. Then, pH and bile salts tolerance were measured in the strains. Molecular analysis of these strains was carried out by PCR-sequencing and their results were used to draw a phylogenetic tree.
Results: Of the 50 isolates, 7 isolates were lactic acid bacteria and two strains were completely probiotic. These strains were resistance to low pH and 0.3% bile salt. Molecular analysis showed that two strains SE and SG were probiotics related to Lactobacillus fermentum (86% homology) and Lactobacillus rhamnosus (61% homology) family, respectively, which were known as new strains.
Conclusion: The results suggested that there are 7 strains with good tolerance in the gastrointestinal tract that have probiotic properties and this is the basis of future studies to evaluate their anti-tumor properties and ultimately oral administration.
Materials and Methods: In this study, out of 60 stool samples collected from healthy children and adult people, 50 bacterial strains were isolated. Antibiotic susceptibility was performed by 15 antibiotics for 7 strains. Then, pH and bile salts tolerance were measured in the strains. Molecular analysis of these strains was carried out by PCR-sequencing and their results were used to draw a phylogenetic tree.
Results: Of the 50 isolates, 7 isolates were lactic acid bacteria and two strains were completely probiotic. These strains were resistance to low pH and 0.3% bile salt. Molecular analysis showed that two strains SE and SG were probiotics related to Lactobacillus fermentum (86% homology) and Lactobacillus rhamnosus (61% homology) family, respectively, which were known as new strains.
Conclusion: The results suggested that there are 7 strains with good tolerance in the gastrointestinal tract that have probiotic properties and this is the basis of future studies to evaluate their anti-tumor properties and ultimately oral administration.
Keywords: New probiotics, Lactobacillus fermentum, Lactobacillus rhamnosus, Molecular analysis, West of Mazandaran Province
Type of Study: Original Research Article |
Subject:
Molecular Microbiology
Received: 2019/02/22 | Accepted: 2019/05/11 | ePublished: 2019/07/22
Received: 2019/02/22 | Accepted: 2019/05/11 | ePublished: 2019/07/22
References
1. Jacouton E, Chain F, Sokol H, Langella P, Bermudez-Humaran LG. Probiotic Strain Lactobacillus Casei BL23 Prevents Colitis-Associated Colorectal Cancer. Frontiers in Immunology. 2017; 8:1553. [DOI:10.3389/fimmu.2017.01553] [PMID] [PMCID]
2. Mattar MC, Lough D, Pishvaian MJ, Charabaty A. Current Management of Inflammatory Bowel Disease and Colorectal Cancer. Gastrointestinal Cancer Research: GCR. 2011; 4(2):53-61.
3. Ciernikova S, Mego M, Semanova M, Wachsmannova L, Adamcikova Z, Stevurkova V, et al. Probiotic Survey in Cancer Patients Treated in the Outpatient Department in a Comprehensive Cancer Center. Integrative Cancer Therapies. 2017; 16(2):188-195. [DOI:10.1177/1534735416643828] [PMID] [PMCID]
4. Goto Y, Kiyono H. Epithelial Barrier: An Interface for the Cross-Communication Between Gut Flora and Immune System. Immunological Reviews. 2012; 245(1):147-163. [DOI:10.1111/j.1600-065X.2011.01078.x] [PMID]
5. Uccello M, Malaguarnera G, Basile F, D'Agata V, Malaguarnera M, Bertino G, et al. Potential Role of Probiotics on Colorectal Cancer Prevention. BMC Surgery. 2012; 12 Suppl 1:S35. [DOI:10.1186/1471-2482-12-S1-S35] [PMID] [PMCID]
6. Zhong L, Zhang X, Covasa M. Emerging Roles of Lactic Acid Bacteria in Protection Against Colorectal Cancer. World Journal of Gastroenterology. 2014; 20(24):7878-7886. [DOI:10.3748/wjg.v20.i24.7878] [PMID] [PMCID]
7. Kahouli I, Tomaro-Duchesneau C, Prakash S. Probiotics in Colorectal Cancer (CRC) With Emphasis on Mechanisms of Action and Current Perspectives. Journal of Medical Microbiology. 2013; 62(Pt 8):1107-1123. [DOI:10.1099/jmm.0.048975-0] [PMID]
8. Bertkova I, Hijova E, Chmelarova A, Mojzisova G, Petrasova D, Strojny L, et al. The Effect of Probiotic Microorganisms and Bioactive Compounds on Chemically Induced Carcinogenesis in Rats. Neoplasma. 2010; 57(5):422-428. [DOI:10.4149/neo_2010_05_422] [PMID]
9. Hendler R, Zhang Y. Probiotics in the Treatment of Colorectal Cancer. Medicines (Basel). 2018; 5(3). [DOI:10.3390/medicines5030101] [PMID] [PMCID]
10. Turner S, Pryer KM, Miao VP, Palmer JD. Investigating Deep Phylogenetic Relationships Among Cyanobacteria and Plastids by Small Subunit rRNA Sequence Analysis 1. Journal of Eukaryotic Microbiology. 1999; 46(4):327-338. [DOI:10.1111/j.1550-7408.1999.tb04612.x] [PMID]
11. Raman M, Ambalam P, Kondepudi KK, Pithva S, Kothari C, Patel AT, et al. Potential of Probiotics, Prebiotics and Synbiotics for Management of Colorectal Cancer. Gut Microbes. 2013; 4(3):181-192. [DOI:10.4161/gmic.23919] [PMID] [PMCID]
12. Soleimani Ff, Ghobadi DM, Piravivanak Z. Screening Local Lactobacilli From Iran in Terms of Production of Lactic Acid and Identification of Superior Strains. Biological Journal of Microorganism. 2015; 4(15):155-166.
13. Melo TA, Dos Santos TF, Pereira LR, Passos HM, Rezende RP, Romano CC. Functional Profile Evaluation of Lactobacillus fermentum TCUESC01: A New Potential Probiotic Strain Isolated during Cocoa Fermentation. BioMed Research International. 2017; 2017:5165916. [DOI:10.1155/2017/5165916] [PMID] [PMCID]
14. Todorov SD, Botes M, Guigas C, Schillinger U, Wiid I, Wachsman MB, et al. Boza, A Natural Source of Probiotic Lactic Acid Bacteria. Journal of Applied Microbiology. 2008; 104(2):465-477.
15. Brink M, Todorov SD, Martin JH, Senekal M, Dicks LM. The Effect of Prebiotics on Production of Antimicrobial Compounds, Resistance to Growth at Low pH and in the Presence of Bile, and Adhesion of Probiotic Cells to Intestinal Mucus. Journal of Applied Microbiology. 2006; 100(4):813-820. [DOI:10.1111/j.1365-2672.2006.02859.x] [PMID]
16. Gupta A SN. Characterization of Potential Probiotic Lactic Acid Bacteria- Pediococcus acidilactici Ch-2 Isolated from Chuli: A Traditional Apricot Product of Himalayan Region for the Production of Novel Bioactive Compounds with Special Therapeutic Properties. J Food Microbiol Saf Hyg. 2017; 2(1):1-11. [DOI:10.4172/2476-2059.1000119]
17. Leite AM, Miguel MA, Peixoto RS, Ruas-Madiedo P, Paschoalin VM, Mayo B, et al. Probiotic Potential of Selected Lactic Acid Bacteria Strains Isolated From Brazilian Kefir Grains. Journal of Dairy Science. 2015; 98(6):3622-3632. [DOI:10.3168/jds.2014-9265] [PMID]
18. Kim SE, Kim YH, Lee H, Kim DO, Kim HY. Probiotic Properties of Lactic Acid Bacteria Isolated From Mukeunji, A Long-Term Ripened Kimchi. Food Science and Biotechnology. 2012; 21(4):1135-1140. [DOI:10.1007/s10068-012-0148-4]
19. Wang SM, Zhang LW, Fan RB, Han X, Yi HX, Zhang LL, et al. Induction of HT-29 Cells Apoptosis by Lactobacilli Isolated From Fermented Products. Research in Microbiology. 2014; 165(3):202-214. [DOI:10.1016/j.resmic.2014.02.004] [PMID]
20. Choi SS, Kim Y, Han KS, You S, Oh S, Kim SH. Effects of Lactobacillus Strains on Cancer Cell Proliferation and Oxidative Stress In Vitro. Letters in Applied Microbiology. 2006; 42(5):452-458. [DOI:10.1111/j.1472-765X.2006.01913.x] [PMID]
21. Fichera GA, Fichera M, Milone G. Antitumoural Activity of a Cytotoxic Peptide of Lactobacillus Casei Peptidoglycan and Its Interaction With Mitochondrial-Bound Hexokinase. Anti-Cancer Drugs. 2016; 27(7):609-619. [DOI:10.1097/CAD.0000000000000367] [PMID] [PMCID]
22. Lam EK, Tai EK, Koo MW, Wong HP, Wu WK, Yu L, et al. Enhancement of Gastric Mucosal Integrity by Lactobacillus Rhamnosus GG. Life Sciences. 2007; 80(23):2128-2136. [DOI:10.1016/j.lfs.2007.03.018] [PMID]
23. Escamilla J, Lane MA, Maitin V. Cell-Free Supernatants From Probiotic Lactobacillus Casei and Lactobacillus Rhamnosus GG Decrease Colon Cancer Cell Invasion In Vitro. Nutrition and Cancer. 2012; 64(6):871-678. [DOI:10.1080/01635581.2012.700758] [PMID]
24. Linsalata M, Cavallini A, Messa C, Orlando A, Refolo MG, Russo F. Lactobacillus Rhamnosus GG Influences Polyamine Metabolism in HGC-27 Gastric Cancer Cell Line: A Strategy Toward Nutritional Approach to Chemoprevention of Gastric Cancer. Current Pharmaceutical Design. 2010; 16(7):847-853. [DOI:10.2174/138161210790883598] [PMID]
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