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:: year 11, Issue 5 (November - December 2017) ::
Iran J Med Microbiol 2017, 11(5): 98-106 Back to browse issues page
Evaluation and Comparison of ELISA and Indirect Immunofluorescence Methods for the Detection of Anti-Listeria Antibodies Among Women with Spontaneous Abortion Referred to Kamali Hospital in Karaj, Iran
Elaheh Tajedini 1, Somayeh Talebi 2, Jalil vandyousefi 3
1- Department of Microbiology, Islamic Azad University, Falavarjan Branch, Isfahan, Iran
2- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran , romin.talebi.1@gmail.com
3- Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Karaj Branch, Karaj, Iran
Abstract:   (1896 Views)
Background and Aims: Listeria monocytogenes is one of the most important causes of abortion and postpartum infection in newborns. This study aimed to compare the indirect ELISA and immunofluorescence methods for the detection of anti-Listeria antibodies among women with spontaneous abortion.
Materials and Methods: In this study, indirect ELISA and immuno-fluorescence methods were used for the detection of anti-Listeria antibodies. Five milliliters of blood sample were collected from mothers with spontaneous abortion during the third trimester of pregnancy in a ward that examines the diseases of female genital tract   in Kamali Hospital of Karaj, Iran. The samples were then transferred to the laboratory for examination using relevant kits. The serum titers of positive samples were also assessed via both ELISA and indirect immunofluorescence for evaluation of IgG and IgM antibodies. The antibodies titers were compared using SPSS software while P values <0.001 were considered as significant.
Results: From the 58 evaluated serum samples, 21 were positive for anti-Listeria antibody. Statistical analysis showed that ELISA and indirect immunofluorescence methods had identical sensitivity. Moreover, it was shown that identification of IgG antibody was better performed   in both diagnostic methods.
Conclusions: Both ELISA and indirect immunofluorescence methods are suitable methods for the detection of antibodies against Listeria.
Keywords: Listeria, ELISA, IFA, abortion
Full-Text [PDF 806 kb]   (730 Downloads)    
Type of Study: Original | Subject: Medical Bacteriology
References
1. Stambach NR, Carr SA, Cox CR, Voorhees KJ. Rapid detection of Listeria by bacteriophage amplification and SERS-Lateral flow immunochromatography. Viruses 2015;7(12):6631-41. [DOI] [PubMed]
2. Abfalter CM, Schmidt TP, Wessler S. Proteolytic activities expressed by gastrointestinal pathogens Bacillus cereus, Listeria monocytogenes and Enterococcus faecium in different growth phases. Br Microbiol Res J 2015; 7(2):62-70. [DOI] [PubMed]
3. Wang Y, Wang Y, Ma A, Li D, Luo L, Liu D, et al. The novel multiple inner primers-loop-mediated isothermal amplification (MIP-LAMP) for rapid detection and differentiation of Listeria monocytogenes. Molecules 2015;20(12):21515-31. [DOI] [PubMed]
4. Soni DK, Singh DV, Dubey SK. Pregnancy - associated human listeriosis: virulence and genotypic analysis of Listeria monocytogenes from clinical samples. J Microbiol 2015;53(9):653-60. [DOI] [PubMed]
5. Pérez-Trallero E, Zigorraga C, Artieda J, Alkorta M, Marimón JM. Two outbreaks of Listeria monocytogenes infection, Northern Spain. Emerg Infect Dis 2014;20(12):2155-7. [DOI] [PubMed]
6. Okpo E, Leith J, Smith-Palmer A, Bell J, Parks D, Browning F,et al. An outbreak of an unusual strain of Listeria monocytogenes infection in North-East Scotland. J Infect Public Health 2015;8(6):612-8. [DOI] [PubMed]
7. Gu Y, Liang X, Huang Z, Yang Y. Outbreak of Listeria monocytogenes in Pheasants. Poult Sci 2015;94(12):2905-8. [DOI] [PubMed]
8. Xiao J, Niu L. Antilisterial peptides released by enzymatic hydrolysis from grass carp proteins and activity on controlling L. monocytogenes inoculated in Surimi Noodle. J Food Sci 2015;80(11):M2564-9. [DOI] [PubMed]
9. Fullerton L, Norrish G, Wedderburn CJ, Paget S, Basu Roy R, Cane C. Nosocomial neonatal Listeria monocytogenes transmission by stethoscope. Pediatr Infect Dis J 2015;34(9):1042-3. [DOI] [PubMed]
10. Benshushan A, Tsafrir A, Arbel R, Rahav G, Ariel L ,Rojansky N.Listeria infection during pregnancy:a 10 years experience.Isr Med Assoc J 2002;4 :776-80. [PubMed]
11. Martne R J .Development of an improved chemically defind minimal for L. monocytogenese .Appl Environ Microbiol 1991; 57(10):3046-8.
12. Mylonakis E, Paliou M, Hohman EL, Calderwood SB, Wing EJ. Listeriosis during pregnancy :a case series and reviwe of 222 case . Medicine (Baltimore) 2002; 81(4):260-9. [DOI]
13. Pujol I, Vidal A, Joven J. The sero prevalent of listeria infection s without clinical sing in a population if pregnant woman from the Reus area .Rev Clin Esp 1990;187(4):175-7. [PubMed]
14. Yaghoobi T, Farshid K, yadollah A M. Listeria monocytogenesis and abortion :a case study of pregnant women in iran . Afr J Microbiol Res 2009; 3(11) :826-32.
15. Mahin J, Abdolreza S, Parivash D, Malihe A, Mehrangiz Z, Fatemeh J. Seropositivity for Listeria monocytogenes in women with Spontaneous abortion . Taiwan Obstet Gynecol 2008; 48 (11):54-61.
16. Vera A, González G, Domínguez M, Bello H. Main virulence factors of Listeria monocytogenes and its regulation. Rev Chilena Infectol 2013;30(4):407-16. [DOI] [PubMed]
17. Wang P, Chen Y, Wang H, Yang S, Xu Y, Li T. A clinical analysis of 16 patients with maternal listeriosis. Zhonghua Nei Ke Za Zhi 2015;54(9):763-7. [PubMed]
18. Chordia N, Sharma NK, Kumar A. An interactomic approach for identification of putative drug targets in Listeria monocytogenes. Int J Bioinform Res Appl 2015;11(4):315-25. [DOI] [PubMed]
19. Pightling AW, Petronella N, Pagotto F. The Listeria monocytogenes core-genome sequence typer (LmCGST): a bioinformatic pipeline for molecular characterization with next-generation sequence data. BMC Microbiol 2015;15:224. [DOI] [PubMed]
20. Gu Y, Liang X, Huang Z, Yang Y. Outbreak of Listeria monocytogenes in Pheasants. Poult Sci 2015;94(12):2905-8. [DOI] [PubMed]
21. Nasrolahei M, Vahedi M. Serologic study of bacterial and viral causes of abortion and fetus death in the patients referring to Imam Khomeini hospital of Sari N orthem Iran. Inter J Molecul Med Adv Sci 2006; 2 (2): 208-10.
22. Sotoodeh Jahromi A, Kazemi A, Manshoori G, Madani A, Moosavy SH, Seddigh B. Seroprevalence of Rubella virus in women with spontaneous abortion. Amer J Infect Dis 2011; 7 (1): 16-9. [DOI]
23. Prieto M, Martínez C, Aguerre L, Rocca MF, Cipolla L, Callejo R. Antibiotic susceptibility of Listeria monocytogenes in Argentina. Enferm Infecc Microbiol Clin 2016; 34(2):91-5. [DOI] [PubMed]
24. Lv J, Qin Z, Xu Y, Xie Q. Listeria infection in Chinese pregnant women and neonates from Shandong. Int J Clin Exp Med 2014;7(9):2730-4. [PubMed]
25. Awofisayo A, Amar C, Ruggles R, Elson R, Adak GK, Mook P, et al. Pregnancy-associated listeriosis in England and Wales. Epidemiol Infect 2015;143(2):249-56. [DOI] [PubMed]
26. Hardy J, Kirkendoll B, Zhao H, Pisani L, Luong R, Switzer A, et al. Infection of pregnant mice with Listeria monocytogenes induces fetal bradycardia. Pediatr Res 2012;71(5):539-45. [DOI] [PubMed]
27. Jadhav S, Bhave M, Palombo EA. Methods used for the detection and subtyping of Listeria monocytogenes. J Microbiol Methods 2012;88(3):327-41. [DOI] [PubMed]
28. Soni DK, Singh DV, Dubey SK. Pregnancy - associated human listeriosis: virulence and genotypic analysis of Listeria monocytogenes from clinical samples. J Microbiol 2015; 53(9):653-60. [DOI] [PubMed]
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Tajedini E, Talebi S, vandyousefi J. Evaluation and Comparison of ELISA and Indirect Immunofluorescence Methods for the Detection of Anti-Listeria Antibodies Among Women with Spontaneous Abortion Referred to Kamali Hospital in Karaj, Iran . Iran J Med Microbiol. 2017; 11 (5) :98-106
URL: http://ijmm.ir/article-1-743-en.html


year 11, Issue 5 (November - December 2017) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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