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:: Volume 11, Number 4 (September - October 2017) ::
Iran J Med Microbiol 2017, 11(4): 13-20 Back to browse issues page
Molecular Detection of Brucella abortus and Brucella melitensis in Raw and Unpasteurized Bulk Cow Milk Tanks of Traditional Domestic Dairy Sale Centres in Khorramabad
Nemat Shams 1, Amin Jaidari2, Lida Etemadfar2
1- Department of Microbiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran , nematshams1386@yahoo.com
2- Department of Microbiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
Abstract:   (114 Views)
Background and Aims: Brucellosis or Malta fever is one of the most prevalent zoonotic diseases considered as a health and economic problem in many countries in the Middle East, including Iran. The consumption of contaminated milk, milk products and contact with infected animals are the main transmission ways of pathogenic Brucella strains among human. The aim of the current study was to determine simultaneous detection of Brucella abortus and Brucella melitensis in raw and none-pasteurized bulk cow milk tanks of traditional domestic dairy sale centres in Khorramabad, Iran.
Materials and Methods: In this cross-sectional study during October and November 2015, a total of 120 samples from raw and unpasteurized bulk cow milk tanks were collected from traditional domestic dairy sale centres in Khorramabad. To confirm the presence of Brucella genus among the samples, single PCR was carried out using B4 and B5 primers and multiplex PCR was then carried out in order to detect the B. abortus and B. melitensis spp..
Results: The present study revealed that 10% of the bulk milk tank samples in traditional domestic dairy sale centres in Khorramabad were contaminated with Brucella.
Conclusions: Results of PCR assay showed that raw and unpasteurized bulk cow milk tanks of traditional domestic dairy sale centres in Khorramabad are the potential cause of human brucellosis in this region. 
 
Keywords: Brucella, Bulk Tank Milk, Khorramabad, PCR
     
Type of Study: Original | Subject: Food Microbiology
References
1. Haque N, Bari MS, Hossain MA, Muhammad N, Ahmed S, Rahman A, et al. An overview of Brucellosis. Mymensingh Med J 2011; 20(4):742–747.
2. Hoffman T, Rock K, Mugizi DR, Muradrasoli S. Molecular detection and characterization of Brucella species in raw informally marketed milk from Uganda. Infect Ecol Epidemiol 2016; 6(1): 1-4.
3. Luna L, Mejía G, Barragán V, Trueba G. Molecular Detection of Brucella Species in Ecuador. Intern J Appl Res Vet Med 2016; 14(2):185-189.
4. Foster G, Osterman BS, Godfroid J, Jacques I, Cloeckaert A. Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. Int J Syst Evol Microbiol 2007; 57(11): 2688-2693.
5. Whatmore AM, Davison N, Cloeckaert A, Al Dahouk S, Zygmunt MS, Brew SD, et al. Brucella papionis sp. nov., isolated from baboons (Papio spp.). Int J Syst Evol Microbiol 2014; 64(12): 4120- 4128.
6. Franco MP, Mulder M, Gilman RH, Smits HL. Human brucellosis. Lancet Infect Dis 2007; 7(12): 775-786.
7. Diaz Aparicio E. Epidemiology of brucellosis in domestic animals caused by Brucella melitensis, Brucella suis and Brucella abortus. Rev Sci Tech 2013; 32(1):53-60.
8. Guerra H. The brucellae and their success as pathogens. Crit Rev Microbiol 2007; 33(4):325-31.
9. Gall D, Nielsen K. Serological diag-nosis of bovine brucellosis: A review of test performance and cost comparison. Rev Sci Tech 2004; 23(3): 989-1002.
10. Baily GG, Krahn JB, Drasar BS, Stoker NG. Detection of Brucella melitensis and Brucella abortus by DNA amplification. J Trop Med Hyg 1992; 95(4): 271-275.
11. Gupta VK, Verma DK, Rout PK, Singh SV, Vihan VS. Polymerase chain reaction (PCR) for detection of Brucella melitensis in goat milk. Small Rumin Res 2006; 65(1-2): 79–84.
12. Hamdy MER, Amin AS. Detection of Brucella Species in the Milk of Infected Cattle, Sheep, Goats and Camels by PCR. Vet J 2002; 163(3): 299-305.
13. Mirnejad R, Mohamadi M, Piranfar V, Mortazavi SM, Kachuei R. Aduplex PCR for the rapid and simultaneous detection of Brucella spp. In human blood samples. Asian pac j trop med 2013; 6(6): 453-456.
14. Ocholi RA, Kwaga JKP, Ajogi I, Bale JOO. Abortion due to Brucella abortus in sheep in Nigeria. Revue Scientifique et Technique 2005; 24(3): 973-979.
15. Shams N, Mansouri F, Jahani E. Seroprevalence of brucellosis in dairy farms of Ivan in 1392. Journal of Zoonoses Research 2013; 2(1): 21-24. [in Persian]
16. Zeinali M, Shirzadi MR, Haj rasuliha H. National Guideline for Brucellosis Control. 2nd ed. Tehran: Ministry of health and medical education; 2012.
17. Bricker BJ, Halling SM. Differentiation of Brucella abortus bv.1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol 1994; 32(11):2660–2666.
18. Dhanashekar R, Akkinepalli S, Nellutla A. Milk-borne infections. An analysis of their potential effect on the milk industry. GERMS 2012; 2(3):101-109.
19. Hashtarkhani S, Akbari M, jarahi L, Atminani K. Epidemiological characteristics and the incidence of brucellosis in the province Khorasan Razavi. Med J Mashhad Univ Med 2015; 58(9): 531-538. [in Persian]
20. Izadi A, Moslemi E, Hossein Yazdi A. Detection of Brucella spp. in raw milk and cheeses samples using hemi nested PCR. New Cell Mol Biotechnol J 2012; 2 (7): 83-90. [in Persian]
21. Shakerian A. Study of contamination rate in craw milk and its traditional products with Brucella abortus, and Brucella mellitensis in Isfahan and Chaharmahal and Bakhtiari Provinces, 2012. J Shahrekord Univ Med Sci 2015; 17(1): 16-23. [in Persian]
22. Shafeie B, Ahmadi M, Dastmalchi Saei H. Diagnosis of Brucella abortus and Brucella melitensis in the Milk of Cattle and Sheep in Kurdistan Province by Polymerase Chain Reaction. jvm 2012; 8(2): 127-135. [in Persian]
23. Khalili M, Aflatoonian M, Salari Aliabadi F, Abshenas J. Brucella contamination in raw milk by polymerase chain reaction. Tehran Univ Med J 2016; 74(7): 517-521. [in Persian]
24. Kangethe EK, Arimi SM, Omore AO, McDermott JJ, Nduhiu JG, Macharia JK, et al. Testing for antibodies to Brucella abortus in milk from consumers and market agents in Kenya using milk ring test and enzyme immunoassay. Kenya Vet 2004; 27(1): 18-21.
25. Gulbaz G, Kamber U. The detection of brucella bacteria with PCR and bacteriological metod in raw milk and some of the dairy products which are consumed in kars. Bull Univ Agric Sci Vet Med Cluj Napoca 2016; 73(1): 127-132.
26. Kaynak-Onurdag F, Okten S, Sent B. Screening Brucella spp. in bovine raw milk by real-time quantitative PCR and conventional methods in a pilot region of vaccination, Edirne, Turkey. J Dairy Sci 2016; 99(5): 3351-3357.
27. Junaidu AU, Oboegbulem SI, Salihu MD. Seroprevalence of brucellosis in prison farm in Sokato, Nigeria. Asia J Epidemiol 2008; 1(1): 24-8.
28. Zowghi E, Ebadi A, Yarahmadi M. Isolation and identification of Brucella organisms in Iran. Iran J of Clin Infect Dis 2008; 3(4): 185-188. [In Persian].
29. Longo M, Mallardo K, Montagnaro S, De Martino L, Gallo S, Fusco G, et al. Shedding of B. abortus rough mutant strain RB51 in milk of water buffalo (Bubalus bubalis). Prev Vet Med 2009; 90(1-2):113–8.
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Shams N, Jaidari A, Etemadfar L. Molecular Detection of Brucella abortus and Brucella melitensis in Raw and Unpasteurized Bulk Cow Milk Tanks of Traditional Domestic Dairy Sale Centres in Khorramabad. Iran J Med Microbiol. 2017; 11 (4) :13-20
URL: http://ijmm.ir/article-1-718-en.html
Volume 11, Number 4 (September - October 2017) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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