year 1, Issue 1 (Spring 2007)                   Iran J Med Microbiol 2007, 1(1): 33-38 | Back to browse issues page

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Saifi M, Pourshafie M, Borhani K, Rahimi F, Soltandallal M. An investigation of aac(6')-le-aph(2")-lagene in MDR and HLGR Enterococcus faecalisand E. faeciumstrains isolated from clinical samples. Iran J Med Microbiol 2007; 1 (1) :33-38
URL: http://ijmm.ir/article-1-71-en.html
1- Soltandallal M.M, Department of Pathobiology, School of Public Health, Medical Sciences
2- Iran Pasteur Institute
3- Soltandallal M.M, Department of Pathobiology, School of Public Health, Medical Sciences , soltanda@tums.ac.ir
Abstract:   (18998 Views)
Background & objectives: Enterococci are important nosocomial pathogens. Multiple drug resistance (MDR) is common among Enterococci and presents difficulties for treatment. High level gentamicin resistance (HLGR) in enterococci, is a significant therapeutic problem. Bactericidal antimicrobial activity usually is obtained by the synergistic combination of a cell wall active agent such as penicillin or glycopeptide with an aminoglycoside. Enterococci can acquire aminoglycoside resistance genes that mediate production of aminoglycoside – modifying enzymes, which eliminate this synergistic bactericidal effect. The most clinically important HLGR genes is aac(6')-le-aph(2")-la.
Material and methods: In the present study , a total of 437 clinical samples from 5 hospital and 3 private laboratory in Tehran , from azar 1384to Tir 1385 , were collected and 300 enterococcal isolates recovered all of the strains were identified to the species level by conventional biochemical tests and assayed for their susceptibility to 11 antibiotics, ampicillin, tetracycline, erythromycin,ciprofloxacin, high dose gentamicin, vancomycin, cotrimoxazole, quinopristin –daifopristin (synercid), linezolid , teicoplanin and nitrofurantoin by disk diffusion method. Gentamicin MIC was accomplished for HLGR strains.
Results: The most frequent species was E. faecalis (81.3%) and then E. faecium (18.7%). MDR strains were detected in 50% and 95% of E.faecalis and E.faecium, respectively. The number of HLGR strains for E.faecalis and E.faecium were found to be 19.5% and 23.5% , respectively. All HLGR strains showed MIC>1024 µg/mL The PCR results showed that 83% and 100% of E.faecalisand E.faecium strains carried aac(6')-le-aph(2")-la gene as detected by PCR.
Conclusion: The present study indicates high rate dissemination of aac(6')-le-aph(2")-la gene, suggesting the possible mechanism of transfer of gentamicin resistant genes within the enterococcal population and in this case probable need to new aminoglycosides orother antibiotics would be predictable
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Type of Study: Original Research Article | Subject: Molecular Microbiology
Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10

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