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:: Volume 11, Number 4 (September - October 2017) ::
Iran J Med Microbiol 2017, 11(4): 35-44 Back to browse issues page
Bioaccumulation of Vitamin D3 by Lactobacillus plantarum and Optimization with Response Surface Methodology
Morteza Mohajeri Amiri1, Mohammad Reza Fazeli2, Nasrin Samadi 3, Mohsen Amini4, Nasim Hayati Roodbari1
1- Department of Biology, Science and Research branch, Islamic Azad University, Tehran, Iran
2- Department of Drug and Food Control, Faculty of Pharmacy and Pharmaceutical Quality Assurance Research Center, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Drug and Food Control, Faculty of Pharmacy and Pharmaceutical Quality Assurance Research Center, Tehran University of Medical Sciences, Tehran, Iran , samadin@tums.ac.ir
4- Department of Medicinal Chemistry, Faculty of Pharmacy and Drug Design, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (91 Views)

Background and Aims: Vitamin D3 deficiency can causes many diseases such as rickets, osteopenia and osteoporosis and increases the risk of some types of cancer. Probiotic strain of Lactobacillus plantarum, which can store fat-soluble vitamin D3 in its bulk, can reduce the effects of vitamin D3 deficiency in addition to being able to produce products with probiotic benefits..
Materials and Methods: This research was carried out in 2016. By designing the one-factor-at-a- time tests, the range of possible effective variables on vitamin D3 absorption in bacterial mass and effective factors were selected. Optimization of vitamin D3 entrapment in biomass of bacteria was performed using response surface methodology via Box-Behnken design. The high-performance liquid chromatography was employed for determination of vitamin D3 quantities.
Results: Among the parameters affecting vitamin D3 entrapment, three factors including incubation temperature, initial vitamin D3 and sucrose concentrations were most effective. The optimal points were obtained at vitamin D3 concentration of 351723.537 IU/mL, sucrose concentration of 2.89 (g/L) and incubation temperature of 33.8 °C. The maximum value of vitamin D3 in dry cell weight of L. plantarum was 1028.5 IU/g which was consistent with the proposed statistical model.
Conclusions: In this study L. plantarum enriched with vitamin D3 was produced and optimized for the first time. Experimental and statistical studies confirm the accuracy and reliability of this optimization.
 

Keywords: Lactobacillus plantarum, Vitamin D3, Box-Behnken design, Response surface methodology
     
Type of Study: Original | Subject: Food Microbiology
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Mohajeri Amiri M, Fazeli M R, Samadi N, Amini M, Hayati Roodbari N. Bioaccumulation of Vitamin D3 by Lactobacillus plantarum and Optimization with Response Surface Methodology. Iran J Med Microbiol. 2017; 11 (4) :35-44
URL: http://ijmm.ir/article-1-706-en.html
Volume 11, Number 4 (September - October 2017) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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