Investigation of Effective Factors on the Optimization of Surface Layer Protein Production in the Deinococcus radiodurans R1 Strain using Response Surface Method

Ebadi Sharaf abad, Behruz; Khalilzadeh, Rassoul; Alijanianzadeh, Mahdi; Abdolirad, Maryam

year 11, Issue 3 (July - August 2017) Iran J Med Microbiol 2017, 11(3): 59-70 | Back to browse issues page

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Ebadi Sharaf abad B, Khalilzadeh R, Alijanianzadeh M, Abdolirad M. Investigation of Effective Factors on the Optimization of Surface Layer Protein Production in the Deinococcus radiodurans R1 Strain using Response Surface Method . Iran J Med Microbiol 2017; 11 (3) :59-70
URL: http://ijmm.ir/article-1-680-en.html

Investigation of Effective Factors on the Optimization of Surface Layer Protein Production in the Deinococcus radiodurans R1 Strain using Response Surface Method

Behruz Ebadi Sharaf abad¹

, Rassoul Khalilzadeh

², Mahdi Alijanianzadeh¹

, Maryam Abdolirad¹

1- Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran
2- Department of Bioscience and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran , rkhalilzadeh@mut.ac.ir

Abstract: (7974 Views)

Background and Aims: Due to its self-assembly properties on variety of surfaces and creating regular functional groups, surface layer protein isolated from bacteria, has significant applications in the field of nanobiotechnology such as biosensor production, targeting drug delivery systems and tissue engineering. In this research, optimization of discontinuous culture medium compositions for the production of HPI surface layer protein from Deinococcus radiodurans R₁ strain was performed using the response surface (RSM) method.
Materials and Methods: In 2016, culture medium for 16 designed experiments with fractional factorial analysis (FFA) was prepared and effective factors among six variables of the discontinuous culture medium was investigated for the production of surface layer proteins of D.radiodurans R₁. Twenty experiments were then designed for the optimization of effective variables using central composite design (CCD) method. Surface protein purity was assessed using SDS-PAGE analysis and its concentration was calculated via Bradford method.
Results: The optimized medium containing 13.36 g/L glucose, 5 g/L yeast extraction, 5 g/L tryptone, 2 g/L HEPES buffer, 0.55 MgSO₄*7H₂O, 0.0368 g/L MnCl₂*4 H₂O, was determined. Wet cellular mass was found as 16.87 g/L, which is 24% more than TGY basic medium and 74% much more than TYG culture medium including NaCl.
Conclusions: Results of the Bradford method demonstrated that the concentration of surface layer protein HPI isolated from D. radiodurans R₁ was 5.6 mg which was more than twice of that using 1liter of basic TGY medium.

Keywords: Deinococcus radiodurans R1, S-layer protein, Response surface methodology (RSM)

Full-Text [PDF 1119 kb] (2441 Downloads)

Type of Study: Original Research Article | Subject: Nanotechnology In Medicine
Received: 2017/03/2 | Accepted: 2017/06/14 | ePublished: 2017/08/8

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