Study the antimicrobial effect of three marine sponges (Dysidea sp.) collected at Persian Gulf on some pathogenic bacteria in planktonic and biofilm forms

Hamayeli, Homa; Hasanshahian, Mehdi; Namaki Shoshtari, Abdolhamid; Askari Hesni, Majid

year 11, Issue 4 (September - October 2017) Iran J Med Microbiol 2017, 11(4): 45-56 | Back to browse issues page

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Hamayeli H, Hasanshahian M, Namaki Shoshtari A, Askari Hesni M. Study the antimicrobial effect of three marine sponges (Dysidea sp.) collected at Persian Gulf on some pathogenic bacteria in planktonic and biofilm forms. Iran J Med Microbiol 2017; 11 (4) :45-56
URL: http://ijmm.ir/article-1-649-en.html

Study the antimicrobial effect of three marine sponges (Dysidea sp.) collected at Persian Gulf on some pathogenic bacteria in planktonic and biofilm forms

Homa Hamayeli¹

, Mehdi Hasanshahian

², Abdolhamid Namaki Shoshtari¹

, Majid Askari Hesni¹

1- Department of Biology, Faculty of Science, Shahid Bahonar of University of Kerman, Kerman, Iran
2- Department of Biology, Faculty of Science, Shahid Bahonar of University of Kerman, Kerman, Iran , hasanshahi@gmail.com

Abstract: (9095 Views)

Background and Aims: Marine sponges have specific ability to growth in complex conditions deal with microorganisms by production of secondary metabolites. These metabolites can be used to degrade and inhibit the formation of microbial biofilms. Eliminating biofilms is important from the industrial and health aspect.
Materials and Methods: In this study the antimicrobial effects of methanol: dichloromethane (1:1) extracts of three marine sponges in Dysidea sp genus collected in 2015 at Persian Gulf, were studied against 6 human pathogenic bacteria. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of each extracts was determined. In biofilm level, the destruction of biofilm and inhibition of biofilm production by each extract was carried out separately.
Results: The results of this study showed that the Dysidea sp_c have the highest inhibitory effect on planktonic form of studied bacteria. The maximum inhibitory effect on biofilm formation related to extracts of Dysidea sp_.a in 12.5 mg/mL concentration against B. cereus and the minimum inhibitory effect in this concentration against P. aeroginosa. The highest destructive biofilm effect of Dysidea sp_.c extract was observed in 6.25 mg/mL against K. pneumonia and lowest destruction in 12.5 mg/mL concentration was recorded against B. cereus.
Conclusions: The results obtained in this research confirmed that each marine sponges have different inhibitory effect against tested bacteria. This difference can be related to production of bioactive compounds and symbiosis microorganisms with these sponges.

Keywords: Sponges, Pathogenic bacteria, Antibiotic resistance, Extracts, Persian Gulf

Full-Text [PDF 789 kb] (2550 Downloads)

Type of Study: Original Research Article | Subject: Antimicrobial Substances
Received: 2016/12/22 | Accepted: 2017/07/24 | ePublished: 2017/09/12

References

1. Bhatnaga I, Kim S. Immense Essence of Excellence: Marine Microbial Bioactive Compounds. MDPI. 2010;2673-701.

2. Hardoim CCP, Costa R. Microbial Communities and Bioactive Compounds in Marine Sponges of the Family Irciniidae. MDPI. 2014;12:5089-122. [DOI]

3. Prem Ananda T, Bhata AW, Yogesh S, Upal Royb S, Siddharthb J, Sarmaa SP. Antimicrobial activity of marine bacteria associated with sponges from the waters off the coast of South East India. Microbiological Research. 2006;161:252-62. [DOI] [PubMed]

4. Javanbakht Yousefi S, Ghasemi FM, Amir Mozaffari N. Biological evaluation of bioactive substances produced in bacteria isolated from coastal waters of the Caspian. Iaulahijan. 2009; 3(4): 2008-5982.

5. Thomas TRA, Kavlekar DP, LokaBharathi PA. Marine Drugs from Sponge-Microbe Association. MDPI. 2010;8:1417-68. [DOI]

6. Newbold RW, Jensen PR, Fenical W, Pawlik JR. Antimicrobial activity of Caribbean sponge extracts. AME. 1999;19:279-84. [DOI]

7. Nazemi M, Pishehvarzad F, Motlebi A, Ahmadzadeh A. Investigation of antibacterial activities of sponge Axinella sinoxea’s extracts from Larak Island, Persian Gulf. JAE Hormozgan. 2012;1(4):65-54.

8. Simo˜es M, Simo˜es L, Vieira MJ. A review of current and emergent biofilm control strategies. LWT - FST. 2010;43:573-83. [DOI]

9. Torkamani S, Shokoh Saremi E. Biofilm Bacterial. Thesis Undergraduate on Food Industry. Khazar Institute of Higher Education. 2013.

10. Yoshida H, Katsuzaki H, Ohta R, Ishikawa K, Fukuda H, Fujino T, Suzuki A. Antimicrobial activity of the thiosufinates isolated from oil macerated garlic extract. JSBBA. 1999;63:591-4.

11. Androw JM. Standardized disc suceptibility testing method. JAC. 2001;25:48-57.

12. Mohajerfar T, Hosseinzadeh A, Akhondzadeh Basti A, Khanjari A, Misaghi A, Gandomi Nasrabadi H. Determination of Minimum Inhibitory Concentration (MIC) of Zataria multiflora Boiss. Essential Oil and Lysozim on L. monocytogenes. Jmp. 2012;11(4):1684-0240.

13. Baron EJ, Finegold SM. (1994) Methods for testing antimicrobial effectiveness. Baily and Scott`s Diagnostic Microbiology. (NY): Mosby Company; 1994.

14. Rosenberg M, Rosenberg E. Role of adherence in growth of Acinetobacter calcoaceticus RAG-1 to hexadecane. JB. 1981;148:51-7.

15. Cramton SE, Gerke C, Cotz F. In vitro methods to study biofilm formation. Methods Enzymology. 2001;336:239-55. [DOI]

16. Jabra-Rizk MA, Meiller TF, James CE, Shirtliff ME. Effect of Farnesol on Staphylococcus aureus biofilm formation and antimicrobial susceptibility. AA. 2006;50:1463-9. [DOI]

17. Sonak S, Bhosle NB. A simple method to assess bacterial attachment to surfaces. JB. 1995;9:31-8. [DOI]

18. Nostro A, Bisignano G, Cannatelli MA, Crisafi G, Germano MP, Alonzo V. Effects of Helichrysum italicum extract on growth and enzymatic activity of Staphylococcus aureus. IJAA. 2001;17:517-20.

19. Aneiros A, Garateix, A. Bioactive peptides from marine sources: pharmacological properties and isolation procedures. JCB. 2004;803:41-53. [DOI] [PubMed]

20. Kelman D, Kashman Y, Rosenberg E, Ilan M, Ifrach I, Loya Y. Antimicrobial activity of the reef sponge Amphimedon viridis from the Red Sea: evidence for selective toxicity. AME. 2001;24:9-16. [DOI]

21. Boopathy S, Kumar TTA, Kathiresan K. Isolation of symbiotic bacteria and bioactive proteins from the marine sponge, Callyspongia diffusa. IJB. 2009;272-5.

22. Govinden-Soulange J, Marie D, Kauroo S, Beesoo R, Ramanjooloo R. Antibacterial Properties of Marine Sponges from Mauritius Waters. TJPR. 2014;13(2):249-54. [DOI]