Frequency of Pathogenic Genes fimH ،irp2 ، rmpA، allS and wcaG of Klebsiella pneumoniae Isolates by Multiplex-PCR Method

Hormozi, Bahman; Rashki, Ahmad; Alipoor, Majid; Najimi, Mohsen

year 11, Issue 6 (January - February 2018) Iran J Med Microbiol 2018, 11(6): 178-183 | Back to browse issues page

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Hormozi B, Rashki A, Alipoor M, Najimi M. Frequency of Pathogenic Genes fimH ،irp2 ، rmpA، allS and wcaG of Klebsiella pneumoniae Isolates by Multiplex-PCR Method. Iran J Med Microbiol 2018; 11 (6) :178-183
URL: http://ijmm.ir/article-1-629-en.html

Frequency of Pathogenic Genes fimH ،irp2 ، rmpA، allS and wcaG of Klebsiella pneumoniae Isolates by Multiplex-PCR Method

Bahman Hormozi¹

, Ahmad Rashki

², Majid Alipoor³

, Mohsen Najimi⁴

1- Department of Biology, Faculty of Basic Sciences, University of Zabol, Zabol, Iran
2- Department of Physiopathology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran , ah_rashki@usal.es
3- Department of Food Hygiene, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
4- Department of Physiopathology, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran

Abstract: (9312 Views)

Background and Aims: Klebsiella pneumoniae that causes bloodstream and urinary tract infections has several virulence factors encoding genes. Factors like the ability of adherence to the host cell surface, presence of sidrophore receptors and regulation of mucoid phenotype are the common virulence ones involved in the pathogenesis of K. pneumoniae. The aim of this study was to determine the frequency of virulence factor encoding genes in K. pneumoniae isolates, collected from hospitalized patients by Multiplex PCR.
Materials and Methods: In this descriptive study, the frequency of genes encoding virulence factor irp2, wcaG, rmpA, rmpA، allS and fimH were evaluated in 100 K. pneumoniae isolates, collected from patients referred to teaching hospitals of Zahedan city by Multiplex PCR.
Results: Among 100 clinical isolates of K. pneumoniae, allS gene had the highest frequency, 90%, and rmpA had the lowest frequency, 6%. The frequency of genes fimH, irp2, wcaG and rmpA were 88%, 87%, 33% and 6% respectively.
Conclusions: The results of this study showed that the allS, irp2 and fimH genes were more frequent among K. pneumoniae isolated from studied patients in Zahedan city. These findings provide important information on the importance of these virulence factors on pathology of urogenital tract infection, management of Klebsiella infection and success of treatment strategies.

Keywords: Klebsiella pneumoniae, Virulence Factors, PCR

Full-Text [PDF 672 kb] (2391 Downloads)

Type of Study: Original Research Article | Subject: Medical Bacteriology
Received: 2016/10/11 | Accepted: 2018/01/11 | ePublished: 2018/03/19

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