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:: Volume 11, Number 2 (May - June 2017) ::
Iran J Med Microbiol 2017, 11(2): 1-8 Back to browse issues page
Identification and assessment of Salmonella typhimurium, infantis and enteritidis serotypes in clinical samples from medical centers of Kerman province
Abolfazl Moghadam1, Shahram Nazarian *2, Jafar Amani3
1- Department of Biology, College of Science, University of Tehran, Tehran, Iran
2- Department of Biology, Faculty of Science, Imam Hossein University, Tehran, Iran , nazarian56@gmail.com
3- Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract:   (819 Views)

Background and Aim: Salmonellosis is one of the important infectious diseases and can be as spread disease between humans and animals that make it essential for identification and detection of Salmonella. Housekeeping genes are typically important genes which are necessary for maintenance and survival of basic cells and can be considered as a gene diagnostic screening bacterial agents. The aim of this study was analysis of Flic, Sdf1 and FljB, housekeeping genes for screening of typhimurium, infantis and enteritidis serovars isolated in Kerman’s hospitals.

Materials and Methods: In a descriptive study from February 2015 to August 2015, 132Salmonella specimens were taken from patients with acute gastroenteritis referred to different medical centers and hospitals in Kerman. The specimens were transferred to microbiology laboratory for identification of Salmonella with serological and bacteriological standard methods. DNA of Salmonella genus strains were extracted by CTAB and, specific primers of housekeeping genes of Salmonella genus (invA) and typhimurium(fliC), infantis(fljB) and, enteritidis(sdfI) serotypes are used in PCR test.

Results: Using PCR technique, the presence of Salmonella genus were confirmed by amplification of invA gene in 130 out of 132 specimens which are identified as a Salmonella by microbiological and biochemical methods (98%). Also results indicating the prevalence of 19% in infantis, 22% in Salmonella typhimurium and 32% in Salmonella enteritidis.

Conclusions: Results showed that the prevalence of Salmonella enteritidis is more than any other serotypes in this region, but as the global statistics, the prevalence of typhimurium and Infantis are increasing.

Keywords: Housekeeping genes, PCR, Salmonella typhimurium, Salmonella infantis, Salmonella enteritidis
Full-Text [PDF 604 kb]   (67 Downloads)    
Type of Study: Original | Subject: Medical Bacteriology
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Moghadam A, Nazarian S, Amani J. Identification and assessment of Salmonella typhimurium, infantis and enteritidis serotypes in clinical samples from medical centers of Kerman province. Iran J Med Microbiol. 2017; 11 (2) :1-8
URL: http://ijmm.ir/article-1-620-en.html
Volume 11, Number 2 (May - June 2017) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
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