year 4, Issue 1 And 2 (9-2010)
Iran J Med Microbiol 2010, 4(1 And 2): 66-73 |
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1- Department of Microbiology, School of Medicine, Kerman University of Medical sciences, Kerman, Iran
2- Department of Microbiology, School of Medicine, Kerman University of Medical sciences, Kerman, Iran , Davoud1362@gmail.com
3- Kerman Physiology Research Center, Vice Chancellor for Research, Kerman University of Medical Sciences, Iran
2- Department of Microbiology, School of Medicine, Kerman University of Medical sciences, Kerman, Iran , Davoud1362@gmail.com
3- Kerman Physiology Research Center, Vice Chancellor for Research, Kerman University of Medical Sciences, Iran
Abstract: (28276 Views)
Background and Objectives: Escherichia coli is the most common cause of nosocomial
infection including urinary tract infections (UTIS). Due to the presence of various resistance
mechanisms against antibacterial agents specially extended spectrum β -lactamases (ESBLs), the
treatment of infections caused by these bacteria is problematic. Since detection of bacterial
resistance and SHV and TEM type ESBLs which are the common type of β - lactamases is
required for a better management of infection caused by these bacteria, the aim of this study was
to determine the antibacterial resistance pattern and the prevalence of bla
SHV and blaTEM
lactamases genes in clinical isolates of E.colifrom urine of hospitalized patient in Kerman.
Material and Methods: This study was performed on 115 E.coli strains isolated from urine specimens of hospitalized patients in 3 hospitals in Kerman. Agar dilution method was used for determination of minimum inhibitory concentration to selected antibacterial agents. ESBLs production was detected by combined disc method. TEM and SHV type β -lactamases were detected by PCR method using plasmid and chromosomal DNA.
Results: Of the total isolated bacteria, 86(74.7%) showed resistance to 3 antibacterial agents from different classes and were considered as multiple drug resistance (MDR). Totally, 76 (66%) of the isolates produced ESBLs. The PCR assay performed on the plasmid and chromosomal DNA, totally 48 (4107%) of the isolates showed TEM, 39 (33.9%) showed SHV, and 20 (17.3%) of the isolates had both TEM and SHV genes. The lowest level of resistance, only in 7 of the isolates (14.7%), was seen towards ceftizoxim. The highest level of resistance was detected to amoxicillin and trimethoprim /sulfamethoxazole which was in the order of 108 (94%) and 110 (95%) isolates, respectively.
Conclusion: MDR trait in urine isolates is common in this area. Due to high resistance to trimethoprim /sulfamethoxazole and amoxicillin, these antibacterial agents are not recommended for treatment of urinary tract infections. In general, the prevalence of TEM gene was found to be much higher than the SHV.
Material and Methods: This study was performed on 115 E.coli strains isolated from urine specimens of hospitalized patients in 3 hospitals in Kerman. Agar dilution method was used for determination of minimum inhibitory concentration to selected antibacterial agents. ESBLs production was detected by combined disc method. TEM and SHV type β -lactamases were detected by PCR method using plasmid and chromosomal DNA.
Results: Of the total isolated bacteria, 86(74.7%) showed resistance to 3 antibacterial agents from different classes and were considered as multiple drug resistance (MDR). Totally, 76 (66%) of the isolates produced ESBLs. The PCR assay performed on the plasmid and chromosomal DNA, totally 48 (4107%) of the isolates showed TEM, 39 (33.9%) showed SHV, and 20 (17.3%) of the isolates had both TEM and SHV genes. The lowest level of resistance, only in 7 of the isolates (14.7%), was seen towards ceftizoxim. The highest level of resistance was detected to amoxicillin and trimethoprim /sulfamethoxazole which was in the order of 108 (94%) and 110 (95%) isolates, respectively.
Conclusion: MDR trait in urine isolates is common in this area. Due to high resistance to trimethoprim /sulfamethoxazole and amoxicillin, these antibacterial agents are not recommended for treatment of urinary tract infections. In general, the prevalence of TEM gene was found to be much higher than the SHV.
Keywords: nosocomial infection, urinary tract infection, ESBLs, Multi drug resistance, Escherichia coli
Type of Study: Original Research Article |
Subject:
Nosocomial infections
Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10
Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10
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