year 4, Issue 1 And 2 (9-2010)
Iran J Med Microbiol 2010, 4(1 And 2): 42-48 |
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Jalalpoor S, Kermanshahi R, Noohi A, Zarkesh Esfahani H. Role of surface layer nanostructure and production of β–lactamase in penicillin resistant Bacillus cereus strains. Iran J Med Microbiol 2010; 4 (1 and 2) :42-48
URL: http://ijmm.ir/article-1-58-en.html
URL: http://ijmm.ir/article-1-58-en.html
1- Department of Food Industrial, Islamic Azad University Shahreza Branch, Isfahan,Iran , shilla.jalalpoor@yahoo.com
2- Department of Biology, Faculty of Basic Sciences, University of Alzahra , Tehran, Iran
3- Departmen of Biology, Faculty of Sciences, University of Tehran, Tehran, Iran
4- Departmen of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
2- Department of Biology, Faculty of Basic Sciences, University of Alzahra , Tehran, Iran
3- Departmen of Biology, Faculty of Sciences, University of Tehran, Tehran, Iran
4- Departmen of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
Abstract: (23672 Views)
Background and Objectives: S-layer in bacteria is composed of proteins that inhibit antibiotics entry into the cells and therefore can lead to substantial increase in pathogenicity. Due to the significant role that hospital staff hands and other surfaces plays in transfer of nosocomial infections, contamination with B. cereus strains capable of producing S-layer and β–lactamase can lead to the spread of antibiotic resistant nosocomial infections. The objective of this investigation was to survey the frequency of S-layer and β–lactamase production in B.cereus hospital isolates. The possible role of S-layer in inhibiting the translocation of penicillin in B.cereus strain isolated from staff hands and hospital surfaces were also investigated.
Material and Methods: This Study was performed during 2005/2007 in Al-Zahra Hospital of Isfahan University. A total of 274 samples from staff hand and hospital surfaces were collected. Bacterial cultures were performed for 16 hr in TSA (Trypticase Soy Agar). The S-layer surface proteins were isolated using electrophoresis with 10X SDS-PAGE. Antibiotic susceptibility tests were performed using Kirby Bauer technique, followed by the detection of β–lactamase production with acidimetric method.
Results: Of the 247 collected samples, the frequency of B.cereus isolation was 26 (9.49%). The 13 B.cereus strains that were isolated from the hospital staff hands, 11 (84.6%) strains and from the 13 B.cereus strains isolated from hospital surfaces, 1 (7/7%) strain produced S-layer nanostructure. The Susceptibility test results indicated that, 11 (92.3%) of the S-layer non-producing B.cereus strains and 12 (100%) of producing strains were resistant to penicillin. The acidimetric test results indicated that all of the S-layer producing B.cereus strains produced β–lactamase.
Conclusion: The higher prevalence of S-layer producing B.cereus strains isolated from hospital staff hands as well as, the much higher rate of penicillin resistance in these isolates may be attributed to the presence of S-layers.
Material and Methods: This Study was performed during 2005/2007 in Al-Zahra Hospital of Isfahan University. A total of 274 samples from staff hand and hospital surfaces were collected. Bacterial cultures were performed for 16 hr in TSA (Trypticase Soy Agar). The S-layer surface proteins were isolated using electrophoresis with 10X SDS-PAGE. Antibiotic susceptibility tests were performed using Kirby Bauer technique, followed by the detection of β–lactamase production with acidimetric method.
Results: Of the 247 collected samples, the frequency of B.cereus isolation was 26 (9.49%). The 13 B.cereus strains that were isolated from the hospital staff hands, 11 (84.6%) strains and from the 13 B.cereus strains isolated from hospital surfaces, 1 (7/7%) strain produced S-layer nanostructure. The Susceptibility test results indicated that, 11 (92.3%) of the S-layer non-producing B.cereus strains and 12 (100%) of producing strains were resistant to penicillin. The acidimetric test results indicated that all of the S-layer producing B.cereus strains produced β–lactamase.
Conclusion: The higher prevalence of S-layer producing B.cereus strains isolated from hospital staff hands as well as, the much higher rate of penicillin resistance in these isolates may be attributed to the presence of S-layers.
Type of Study: Original Research Article |
Subject:
Nosocomial infections
Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10
Received: 2013/11/10 | Accepted: 2013/11/10 | ePublished: 2013/11/10
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