year 10, Issue 4 (September - October 2016)                   Iran J Med Microbiol 2016, 10(4): 10-16 | Back to browse issues page

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Hajiahmadi F, Safari N, Alijani P, Mordaddi A, Arabestani M R. The frequency of integrons of antibiotic resistant in Stenotrophomonas maltophilia isolates in Hamadan/Iran. Iran J Med Microbiol. 2016; 10 (4) :10-16
URL: http://ijmm.ir/article-1-536-en.html
1- Department of Microbiology,Hamadan University of Medical Sciences, Hamadan, Iran
2- Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran , mr_arabestani@yahoo.com
Abstract:   (5401 Views)

Background and Aim: Stenotrophomonasmaltophilia is an important multidrug-resistant nosocomial pathogen that leads to respiratory tract infections and urinary tract. Due to the increasing of antibiotic resistance in S. maltophilia, treatment of infections caused by these bacteria has been difficult. The aims of this study was detection of antibiotic resistance pattern and identify of class I and II integrons and associated gene cassettes in clinical isolates of S. maltophilia.

Materials and Methods: In this cross-sectional study, of 246 clinical specimen were collected from educational hospitals of Hamadan university of medical sciences, 12 isolates of
S. maltophilia were collected in 2015. After the culture, isolates were verified by standard biochemical methods. Antibiotic susceptibility was determined against 13 antibiotics. Presence of Class I and II integrons genes were tested using specific primers by PCR.

Results: In the pattern of antibiotic resistance, the highest resistance rate showed to Cefditoren, Ceftriaxone, Cefotaxime antibiotics and the lowest resistance to Amikacin, Imipenem, Ofloxacin, Trimethoprim-Sulphamethoxazole and Ciprofloxacin antibiotics were reported. All S. maltophilia strains carried class I integrons. No isolate carry class II integrons and gene cassettes.

Conclusions: The result of this study indicates a high prevalence of class I integrons in
S.maltophilia isolates. Thus, identification of these resistance genes for infection control programs and to prevent the spread of resistant strains is very important.

Full-Text [PDF 424 kb]   (1231 Downloads)    
Type of Study: Original | Subject: Medical Bacteriology
Received: 2016/03/10 | Accepted: 2016/07/25

References
1. Naidu P, Smith S. A review of 11 years of Stenotrophomonas maltophilia blood isolates at a tertiary care institute in Canada. Can J Infect Dis Med Microbiol. 2012;23(4):165-9. [PubMed]
2. Falagas ME, Kastoris AC, Vouloumanou EK, Rafailidis PI, Kapaskelis AM, Dimopoulos G. Attributable mortality of Stenotrophomonas maltophilia infections: a systematic review of the literature. Future microbiol 2009;4(9):1103-9. [PubMed]
3. Brooke JS. Stenotrophomonas maltophilia: an emerging global opportunistic pathogen. Clin Microbiol Rev 2012;25(1):2-41. [PubMed]
4. Brooke JS. Stenotrophomonas maltophilia: an emerging global opportunistic pathogen. Clin Microbiol Rev 2012;25(1):2-41.
5. Hall RM, Collis CM. Mobile gene cassettes and integrons: capture and spread of genes by site specific recombination. Mol Microbiol 1995;15(4):593-600.
6. Farkas A, Crăciunaş C, Chiriac C, Szekeres E, Coman C, Butiuc-Keul A. Exploring the Role of Coliform Bacteria in Class 1 Integron Carriage and Biofilm Formation During Drinking Water Treatment. Microb Ecol 2016; 72(4):1-10. [PubMed]
7. Koczura R, Mokracka J, Barczak A, Krysiak N, Kaznowski A. Association between the presence of class 1 integrons, virulence genes, and phylogenetic groups of Escherichia coli isolates from river water. Microb Ecol 2013;65(1):84-90. [PubMed]
8. Khosravi Y, Tay ST, Vadivelu J. Analysis of integrons and associated gene cassettes of metallo-β-lactamase-positive Pseudomonas aeruginosa in Malaysia. J Med Microbiol 2011;60(7):988-94. [PubMed]
9. Sobia F, Shahid M, Jamali S, Khan H, Niwazi S. Molecular Profiling and Characterization of Integrons and Genotyping of Escherichia coli and Klebsiella pneumoniae Isolates Obtained from North Indian Tertiary Care Hospital. SM Trop Med J. 2016;1(1):1003.
10. Cockerill FR. Performance standards for antimicrobial susceptibility testing: twenty-first informational supplement: Clinical and Laboratory Standards Institute (CLSI); 2011.
11. Neela V, Rankouhi SZR, van Belkum A, Goering RV, Awang R. Stenotrophomonas maltophilia in Malaysia: molecular epidemiology and trimethoprim–sulfamethoxazole resistance. Int J Infect Dis 2012; 16(8):e603-e7. [PubMed]
12. Flores-Trevino S, Gutierrez-Ferman JL, Morfín-Otero R, Rodríguez-Noriega E, Estrada-Rivadeneyra D, Rivas-Morales C, et al. Stenotrophomonas maltophilia in Mexico: antimicrobial resistance, biofilm formation and clonal diversity. J Med Microbiol 2014;63(11):1524-30. [PubMed]
13. Wang WS, Liu CP, Lee C-M, Huang FY. Stenotrophomonas maltophilia bacteremia in adults: four years' experience in a medical center in northern Taiwan. J Microbiol Immunol Infect 2004; 37(6):359-65. [PubMed]
14. Rhee J-Y, Choi JY, Choi M-J, Song J-H, Peck KR, Ko KS. Distinct groups and antimicrobial resistance of clinical Stenotrophomonas maltophilia complex isolates from Korea. J Med Microbiol 2013; 62(5):748-53. [PubMed]
15. Nazik H, Ongen B, Erturan Z, Salcioglu M. Genotype and antibiotic susceptibility patterns of Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolated from cystic fibrosis patients. Jpn J Infect Dis 2007; 60(2-3):82-6. [PubMed]
16. Goncalves-Vidigal P, Grosse-Onnebrink J, Mellies U, Buer J, Rath P-M, Steinmann J. Stenotrophomonas maltophilia in cystic fibrosis: improved detection by the use of selective agar and evaluation of antimicrobial resistance. J Cyst Fibros 2011; 10(6):422-7. [PubMed]
17. Chang LL, Lin HH, Chang CY, Lu PL. Increased incidence of class 1 integrons in trimethoprim/sulfamethoxazole-resistant clinical isolates of Stenotrophomonas maltophilia. J Antimicrob Chemother 2007; 59(5):1038-9. [PubMed]
18. S-J, Lee YL, Hsueh PR. Multidrug resistance in clinical isolates of Stenotrophomonas maltophilia: roles of integrons, efflux pumps, phosphoglucomutase (SpgM), and melanin and biofilm formation. Int J Antimicrob Agents 2010; 35(2):126-30.
19. Chung HS, Kim K, Hong SS, Hong SG, Lee K, Chong Y. The sul1 gene in Stenotrophomonas maltophilia with high-level resistance to trimethoprim/sulfamethoxazole. Ann Lab Med 2015; 35(2):246-9. [PubMed]
20. Hu LF, Chang X, Ye Y, Wang ZX, Shao YB, Shi W, et al. Stenotrophomonas maltophilia resistance to trimethoprim/sulfamethoxazole mediated by acquisition of sul and dfrA genes in a plasmid-mediated class 1 integron. nt J Antimicrob Agents 2011;37(3):230-4. [PubMed]
21. Koeleman JG, Stoof J, Van Der Bijl MW, Vandenbroucke-Grauls CM, Savelkoul PH. I dentification of epidemic strains of Acinetobacter baumannii by integrase gene PCR. J Clin Microbiol 2001;39(1):8-13. [PubMed]
22. Levesque C, Piche L, Larose C, Roy PH. PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob Agents Chemother 1995;39(1):185-91. [PubMed]

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