[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: year 11, Issue 4 (September - October 2017) ::
Iran J Med Microbiol 2017, 11(4): 64-69 Back to browse issues page
Determination of Antibiotic Resistance and Identification of Tetracycline (tet) Resistance Genes in Salmonella enteritidis Strains Isolated from Food Sources
Somayeh Naghizadeh 1, Gholamali Moradl 2
1- Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran
2- Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran , Moradli.mic@gmail.com
Abstract:   (1783 Views)

Background and Aims: Salmonellosis is an important disease among human animals and which is caused by different serovars of Salmonella enterica. Serovars enteritidis and typhimurium are the most prevalent serovars among human and animals which are the causes of foodborne infections. This study aimed to detect the antibiotic resistance pattern in Salmonella strains isolated from red meat as well as the molecular identification of tetracycline resistance genes.
Materials and Methods: In this study, 230 red meat samples were collected during 2015 from slaughterhouses,distribution centers and industrial processing plants around Tehran and were examined using culture and biochemical tests for the presence of Salmonella isolates. Serotyping was performed using O and H antisera. Antimicrobial susceptibility testing was done for all the strains. Detection the tetracycline resistance gene was performed using Multiplex PCR and specific primers for the tetA, tetB, tetC and tetD genes.
Results and Conclusions: A total of 60 Salmonella samples were isolated and identified. The serotyping results showed that all of the 60 samples belonged to group D and serovar enteritidis. The highest resistance was against amoxicillin and the lowest resistance against gentamicin. Twenty five percent of the samples showed phenotypical resistance to tetracycline, among which 60% had the tet genes, 7 isolates (46.6%) having the tetA gene, one isolate having the tetB and one isolate possessing the tetC gene. This study showed the prevalence of Salmonella isolates in red meat samples and and the presence of tetracycline resistance genes among these isolates.

Keywords: Salmonella Typhimurium, Salmonella Enteritidis, tetracycline resistance genes ‎
Full-Text [PDF 548 kb]   (588 Downloads)    
Type of Study: Brief report | Subject: Food Microbiology
References
1. ‎1-‎ Egli T, Koster W, Meile L. Pathogenic microbes in water and food: changes and challenges. FEMS Microbiol Rev. ‎‎2002;26(2):111–12. ‎ [DOI] [PubMed]
2. ‎2-‎ Tacket CO, Narain JP, Sattin R, Lofgren JP, Konigsberg C JR, Rend‌torff RC. A multistate outbreak of infections caused ‎by Yersinia enterocolitica transmitted by pasteurized milk. JAMA. 1984;251(4):483–86. ‎ [DOI] [PubMed]
3. ‎3-‎ Cortez ALL, Carvalho ACFB, Ikunob AA, Burger KP, Vidal-Martins AMC. Identification of Salmonella spp. Isolates ‎from chicken abattoirs by multiplex-PCR.‎‌ ‌Vet Sci. 2006;81: 340-344.‎ [DOI] [PubMed]
4. ‎4-‎ Braden Christopher R. Salmonella enterica serotype Enteritidis and egg; a national apidemic in the united states.‎‌ ‌Food ‎safety. 2006;43: 512-17.‎
5. ‎5-‎ Soltan Dallal MM, Sharifi Yazdi MK, Mirzaei N, Kalantar E. Prevalence of Salmonella spp. in packed and unpacked red ‎meat and chicken in South of Tehran. Jundishapur J Microbiol. 2014; 7(4):9254-58.‎
6. ‎6-‎ Schwarz S and Chaslus-Dancla E. Use of antimicrobials‌ ‌in veterinary medicine and mechanisms of‌ ‌resistance. Vet Res. ‎‎2001;32:201-225.‎ [DOI] [PubMed]
7. ‎7-‎ Shahada F, Chuma T, Tobata T, Okamoto K, Sueyoshi‌ ‌M, Takase K. Molecular epidemiology ofantimicrobial resistance ‎among Salmonella enterica serovar‌ ‌Infantis from poultry in Kagoshima, Japan. Int J Antimicrob Agents. 2006;28:302-7.‎ [DOI] [PubMed]
8. ‎8-‎ Clinical and Laboratory Standards Institute (CLSI).‎‌ ‌Performance standards for antimicrobial susceptibility‌ ‌testing; twenty ‎first informational supplement. M100-S21.Wayne, PA: CLSI. 2011. ‎
9. ‎9-‎ Su HC, Ying GG, Tao R, Zhang RQ, Fogartyb LR.Occurrence of antibiotic resistance and characterization of resistance ‎genes and integrons in Enterobacteriaceae isolated from integrated fish farms in south China. 2011;13: 3229-36.‎
10. ‎10-‎ Soltan Dallal MM, Taremi M, Gachkar L, Modarressi S, Sanaei M, Bakhtiari R. Characterization of antibiotic resistant ‎pat‌terns of Salmonella serotypes isolated from beef and chicken samples in Tehran. Jundishapur J Microbiol. ‎‎2009;2(4):124- 31. ‎
11. ‎11-‎ Jalali M, Abedi D, Pourbakhsh SA, Ghoukasini K. prevalence of Salmonella spp. in raw and cooked foods in Isfahan-‎Iran. J Food Safety. 2008;28:442-52.‎ [DOI]
12. ‎12-‎ Salyers AA and Whitt DD. Revenge of the Microbes: How bacterial resistance is undermining the antibiotic miracle. ‎ASM Press, Washington, DC; 2005.‎
13. ‎13-‎ Ranjbar, R, Naghoni A. Class 1 integron-mediated antibiotic resistance in salmonella enterica strains isolated in Tehran, ‎Iran. Iran J med Microbiol. 2014;7(4): 16-23. (Full text in Persian)‎
14. ‎14-‎ Soltan-Dallal MM, Rahimi Forushani, A., Sadigh Maroufi, S., Sharifi Yazdi, K. The comparison of PCR technique and ‎API-20E kit with the conventional biomedical methods for the identification of Salmonella species in laboratory. Med Lab ‎J. 2011;5(2): 21-27. (Full text in Persian)‎
15. ‎15-‎ Frech G and Schwarz S. Molecular analysis of tetracycline resistance in Salmonella enterica subsp. enterica serovars ‎Typhimurium, Enteritidis, Dublin, Choleraesuis, Hadar and Saintpaul: construction and application of specific gene ‎probes. J Appl Microbiol. 200;89: 633-41.‎
16. ‎16-‎ Tajbakhsh M, Hendriksen RS, Nochi Z, Zali M, Aarestrup FM, Garcia-Migura L. Antimicrobial resistance in Salmonella ‎spp. recovered from patients admitted to six different hospitals in Tehran, Iran from 2007 to 2008. Folia Microbiol. ‎‎2012;57: 91-97.‎ [DOI] [PubMed]
17. ‎17-‎ Carraminana JJ, Rota C, Agustin I, Herrera A. High prevalence of multiple antibiotics in Salmonella serovars from a ‎poultry slaughterhouse in Spain.Vet Microbiol. 2004;104(1-2): 133-9. ‎ [DOI] [PubMed]
18. ‎18-‎ Randall LP, Cooles SW, Osborn MK, Piddock LJV, Woodward MJ. Antibiotic resistance genes, integrons and multiple ‎antibiotic resistance in thirty-five serotypes of Salmonella enterica isolated from humans and animals in the UK. J ‎Antimicrob Chemother. 2004; 53: 208-16.‎
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Naghizadeh S, Moradl G. Determination of Antibiotic Resistance and Identification of Tetracycline (tet) Resistance Genes in Salmonella enteritidis Strains Isolated from Food Sources. Iran J Med Microbiol. 2017; 11 (4) :64-69
URL: http://ijmm.ir/article-1-535-en.html


year 11, Issue 4 (September - October 2017) Back to browse issues page
مجله میکروب شناسی پزشکی ایران Iranian Journal of Medical Microbiology
Persian site map - English site map - Created in 0.06 seconds with 31 queries by YEKTAWEB 3781