The evaluation of Methanolic and aqueous extracts effect of Zizyphus spina-Christi against Leishmania major (MHOM/IR/75/ER) promastigotes using MTT assay

Albakhit, Sedigheh; khademvatan, Shahram; Doudi, Monir

year 10, Issue 3 (July - August 2016) Iran J Med Microbiol 2016, 10(3): 54-60 | Back to browse issues page

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Albakhit S, khademvatan S, Doudi M. The evaluation of Methanolic and aqueous extracts effect of Zizyphus spina-Christi against Leishmania major (MHOM/IR/75/ER) promastigotes using MTT assay. Iran J Med Microbiol 2016; 10 (3) :54-60
URL: http://ijmm.ir/article-1-459-en.html

The evaluation of Methanolic and aqueous extracts effect of Zizyphus spina-Christi against Leishmania major (MHOM/IR/75/ER) promastigotes using MTT assay

Sedigheh Albakhit¹

, Shahram Khademvatan

², Monir Doudi¹

1- Cellular and Molecular Research Center& Department of Medical Parasitology and Mycology Urmia University of Medical Sciences, Urmia, Iran
2- Department of Microbiology, Falavarjan Branch,Islamic Azad University, Falavarjan ,Isfahan , Iran , khademvatan@yahoo.com

Abstract: (8936 Views)

Background and Aim: Leishmaniasis is a zoonotic disease with high prevalence. Various medicines are used for treatment of Leishmaniasis but because of their side effects and drug resistance, the medical society seeks new effective compounds. The aim of this study was the evaluation of Zizyphus spina- Christi as source of new medicinal factor.

Materials and Methods: The methanolic extract was prepared by percolation method. Leishmania major promastigotes cultured at 25±2°C in stationary phase in RPMI-1640 medium complemented with 10% fetal calf serum (FCS) and antibiotic Penicillin-Streptomycin (10,000 U/mL). By using MTT assay, the biological activity of plant extracts was evaluated in comparison with Glucantime against L.major promastigotes and IC50 values (50% inhibitory concentrations) were also studied. All tests were repeated for three times.

Results: Zizyphus spina- Christi extracts and glucantime inhibited the growth of promastigote forms of L.major in vitro after 24, 48 and 72 hours of incubation. IC50 of glucantime was 21.96μg/ml, and IC50 values for methanol and the aqueous extracts of Zizyphus spina- Christi were 60 μg/ml and 80 μg/ml, respectively. All plant extracts had profound effects on promastigotes of L.major.

Conclusions: As regards, methanol and aqueous extract of Zizyphus spina-Christi have anti-leishmanial effects in vitro, further works are required to appraise the exact effect of these extracts on Leishmania agents in animal models.

Keywords: Leishmania major, Zizyphus spina- Christi, plant extract, MTT

Full-Text [PDF 542 kb] (4075 Downloads)

Type of Study: Original Research Article | Subject: Medical Parasitology
Received: 2015/08/1 | Accepted: 2016/01/23 | ePublished: 2016/10/16

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