Relationship between presence of genes encoding ESBLs and antimicrobial susceptibility pattern in Escherichia coli clinical isolates

Mirzaee, Mohsen; Eftekhari, Roohangiz; Taghizadeh, Neda; Mehrabi, Mohammad Reza

year 10, Issue 1 (March - April 2016) Iran J Med Microbiol 2016, 10(1): 8-15 | Back to browse issues page

Mendeley

Zotero

RefWorks

Mirzaee M, Eftekhari R, Taghizadeh N, Mehrabi M R. Relationship between presence of genes encoding ESBLs and antimicrobial susceptibility pattern in Escherichia coli clinical isolates. Iran J Med Microbiol 2016; 10 (1) :8-15
URL: http://ijmm.ir/article-1-377-en.html

Relationship between presence of genes encoding ESBLs and antimicrobial susceptibility pattern in Escherichia coli clinical isolates

Mohsen Mirzaee

¹, Roohangiz Eftekhari²

, Neda Taghizadeh³

, Mohammad Reza Mehrabi⁴

1- Department of Laboratory Sciences, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran , mohsen1439@yahoo.com
2- Microbiology ward, Emmam khomeni Hospital. Boroujerd, Iran.
3- Department of Microbiology, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran.
4- Department of Laboratory Sciences, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran

Abstract: (11399 Views)

Background and aims: Escherichia coli harboring extended spectrum β-lactamase (ESBLs) are significantly resistant to other antibiotics and caused several health problems. The present study was carried out to characterize the ESBL types and to determine their pattern of resistance to four antimicrobial agents in clinical isolates of E. coli.

Material and methods: During April 2012 to May 2013, 500 clinical isolates of E. coli were collected from two hospitals in Boroujerd. Phenotypic screening and confirmation tests for ESBL detection and antibiotic susceptibility testing were performed according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Minimum inhibitory concentration of imipenem, meropenem, amikacin and ceftriaxone was determined by the E-test method. All ESBLs producing isolates were examined for presence of the TEM, SHV and CTX-M genes by PCR method.

Results: All of the studied isolates were susceptible to imipenem and meropenem. Additionally, among ESBLs producer isolates good susceptibility to amikacin and ceftriaxone (P-value ˂ 0.5) was observed. The prevalence of the TEM, SHV and CTX-M genes among 190 ESBLs producing isolates was as follows: TEM positive: 61 (32.1%), SHV positive: 60 (31.6%) and CTX-M: 62 (32.6%). TEM, SHV and CTX-M genes occurred together in 25 (13.1%) of the isolates.

Conclusion: The high prevalence of E.coli producing ESBL in Boroujed hospitals reported. More than one ESBL was produced by many isolates, and this was correlated with increased resistance levels. Carbapenems continue to show good in vitro activity agent ESBLs producing organism.

Keywords: Escherichia coli, Beta-lactamases, Carbapenems

Full-Text [PDF 659 kb] (4398 Downloads)

Type of Study: Original Research Article | Subject: Antibiotic Resistance
Received: 2014/11/14 | Accepted: 2015/02/18 | ePublished: 2016/05/12

References

1. Mirzaee M, Owlia P, Mansouri S. Distribution of CTX-M β-lactamase genes among Escherichia coli strains isolated from patients in Iran. Lab Medicine. 2009;40(12):724-7. [Article]

2. Manoharan A, Premalatha K, Chatterjee S, Mathai D, Group SS. Correlation of TEM, SHV and CTX-M extended-spectrum beta lactamases among Enterobacteriaceae with their in vitro antimicrobial susceptibility. Indian journal of medical microbiology. 2011;29(2):161. [PubMed]

3. Paterson DL, Bonomo RA. Extended-spectrum β-lactamases: a clinical update. Clinical microbiology reviews. 2005;18(4):657-86. [PubMed]

4. Osawa K, Shigemura K, Shimizu R, Kato A, Kusuki M, Jikimoto T, et al. Molecular Characteristics of Extended-Spectrum β-Lactamase–Producing Escherichia coli in a University Teaching Hospital. Microbial Drug Resistance. 2014. [PubMed]

5. Roschanski N, Fischer J, Guerra B, Roesler U. Development of a Multiplex Real-Time PCR for the Rapid Detection of the Predominant Beta-Lactamase Genes CTX-M, SHV, TEM and CIT-Type AmpCs in Enterobacteriaceae. PloS one. 2014;9(7):e100956. [Article]

6. Pitout JD. Infections with extended-spectrum β-lactamase-producing Enterobacteriaceae. Drugs. 2010;70(3):313-33. [PubMed]

7. Bradford PA, Urban C, Mariano N, Projan SJ, Rahal JJ, Bush K. Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC beta-lactamase, and the foss of an outer membrane protein. Antimicrobial agents and chemotherapy. 1997;41(3):563-9. [PubMed]

8. Yesmin T, Hossain M, Paul S, Mahmud C, Kabir M, Haque N, et al. Prevalence and Antimicrobial Susceptibility Pattern of ESBL Producing Isolates. Mymensingh medical journal: MMJ. 2013;22(4):625-31. [Article]

9. Performance standards for antimicrobial susceptibility testing; 21th informational supplement. Clinical and Laboratory Standards Institute Wayne PM-S.

10. Mirzaee M, Pourmand M, Chitsaz M, Mansouri S. Antibiotic Resistance to Third Generation Cephalosporins Due to CTX-M-Type Extended-Spectrum??-Lactamases in Clinical Isolates of Escherichia coli. Iranian Journal of Public Health. 2009;38(1):10-7. [Article]

11. Hanson ND, Thomson KS, Moland ES, Sanders CC, Berthold G, Penn RG. Molecular characterization of a multiply resistant Klebsiella pneumoniae encoding ESBLs and a plasmid-mediated AmpC. Journal of antimicrobial chemotherapy. 1999;44(3):377-80. [PubMed]

12. Zaniani FR, Meshkat Z, Nasab MN, Khaje-Karamadini M, Ghazvini K, Rezaee A, et al. The Prevalence of TEM and SHV Genes among Extended-Spectrum Beta-Lactamases Producing Escherichia Coli and Klebsiella Pneumoniae. Iranian journal of basic medical sciences. 2012;15(1):654. [PubMed]

13. Melzer M, Petersen I. Mortality following bacteraemic infection caused by extended spectrum beta-lactamase (ESBL) producing< i> E. coli compared to non-ESBL producing< i> E. coli. Journal of Infection. 2007;55(3):254-9. [PubMed]

14. Mansouri S, Neyestanaki DK, Shokoohi M, Halimi S, Beigverdi R, Rezagholezadeh F, et al. Characterization of AmpC, CTX-M and MBLs types of β-lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli producing Ex-tended Spectrum β-lactamases in Kerman, Iran. Jundishapur Journal of Microbiology. 2014;7(2). [PubMed]

15. Moghaddam MN, Beidokhti MH, Jamehdar SA, Ghahraman M. Genetic properties of blaCTX-M and blaPER β-lactamase genes in clinical isolates of Enterobacteriaceae by polymerase chain reaction. Iranian journal of basic medical sciences. 2014;17(5):378. [PubMed]

16. Ghasemi Y, Archin T, Kargar M, Mohkam M. A simple multiplex PCR for assessing prevalence of extended-spectrum β-lactamases producing< i> Klebsiella pneumoniae in Intensive Care Units of a referral hospital in Shiraz, Iran. Asian Pacific journal of tropical medicine. 2013;6(9):703-8. [PubMed]

17. Patel TS, Nagel J. Clinical Outcomes of Enterobacteriaceae Infections Stratified by Carbapenem Minimum Inhibitory Concentrations. Journal of clinical microbiology. 2014:JCM. 03057-14. [Article]

18. Kazemnia A, Ahmadi M, Dilmaghani M. Antibiotic Resistance Pattern of Different Escherichia coli Phylogenetic Groups Isolated from Human Urinary Tract Infection and Avian Colibacillosis. Iranian biomedical journal. 2014;18(4):219-24. [PubMed]

19. Moayednia R, Shokri D, Mobasherizadeh S, Baradaran A, Fatemi SM, Merrikhi A. Frequency assessment of β-lactamase enzymes in Escherichia coli and Klebsiella isolates in patients with urinary tract infection. Journal of Research in Medical Sciences. 2014:S41. [PubMed]

20. Peerayeh SN, Rostami E, Siadat SD, Derakhshan S. High Rate of Aminoglycoside Resistance in CTX-M-15 Producing Klebsiella pneumoniae Isolates in Tehran, Iran. Lab Medicine. 2014;45(3):231-7. [PubMed]

21. Livermore DM, Sefton AM, Scott GM. Properties and potential of ertapenem. Journal of Antimicrobial Chemotherapy. 2003;52(3):331-44. [PubMed]