year 4, Issue 3 (Fall 2010)
Iran J Med Microbiol 2010, 4(3): 21-28 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Amini A, Issazadeh K, Rahimi Alang S, Vaez H, Bakhshandeh Nosrat S, Cheraghali F et al . Comparison of sugars fermentation in methicillin resistant and sensitive Staphylococcus aureus isolates. Iran J Med Microbiol 2010; 4 (3) :21-28
URL: http://ijmm.ir/article-1-32-en.html
URL: http://ijmm.ir/article-1-32-en.html
Abolfazl Amini1 
, Khosro Issazadeh1 , Somaye Rahimi Alang1 , Hamid Vaez2 , Sepideh Bakhshandeh Nosrat3 , Fatemeh Cheraghali3 , Ezatallah Ghaemi 3
, Khosro Issazadeh1 , Somaye Rahimi Alang1 , Hamid Vaez2 , Sepideh Bakhshandeh Nosrat3 , Fatemeh Cheraghali3 , Ezatallah Ghaemi 3
1- Department of Microbiology, Islamic Azad University, Lahijan branche, Iran
2- Department of Microbiology, Golestan University of Medical Sciences, Golestan, Iran
3- Center for Infection Research, Golestan University of Medical Sciences, Golestan, Iran
2- Department of Microbiology, Golestan University of Medical Sciences, Golestan, Iran
3- Center for Infection Research, Golestan University of Medical Sciences, Golestan, Iran
Abstract: (16559 Views)
Background and objectives: The mec A gene in Staphylococcus aureus leads to production of new penicillin-binding protein called PBP2a. This phenomenon may change some phenotypic properties. The aim of this study was the comparison of sugars fermentation in MRSA (Methicillin resistant Staphylococcus aureus) and MSSA (Methicillin sensitive Staphylococcus aureus) isolates.
Material and Methods: Total 206 MRSA and MSSA isolates (57 MRSA and 149 MSSA isolates) obtained from 120 inpatients and 86 healthy carriers in the Gorgan were tested. Sugar fermentation carried out in phenol red broth medium, containing glucose, galactose, arabinose, fructose, xylose, rhamnose, mannose, sucrose, trehalose, raffinose or maltose . Change in color after 24 hours incubation at 37°C were recorded. The data were analyzed by SPSS software, using Chi squared test and P<0.05 was considered as meaningful.
Results: The fermentation of glucose at the 4th hour of incubation happened in 10(17.5%) and 11(7.4%) of MRSA and MSSA isolates respectively (P=0.03). The ability ferment rhamnose and xylose in MRSA isolates were 11 (19.3%) and 6 (10.5%), and in MSSA strains were 3 (2%) and 4 (2.7%) respectively. The differences between MRSA and MSSA isolates was statistically significant (P<0.05). The highest difference between S.aureus which isolated from patients and carriers were seen in fermentation of fructose [120 (100%) vs 80 (93%)], raffinose [46 (38.3%) vs 2 (2.3%)] and rhamnose [14 (11.7%) vs 0 (0%)] (P<0.005). The ability of S.aureus isolates with ability of raffinose fermentation isolated from urinary tract infections was 23 (%56.1), which is significant (P=0.03).
Conclusion: Methicillin resistant in Staphylococcus aureus isolates is accompanied with increased ability of sugars fermentation. The ability of sugars fermentation in Staphylococcus aureus isolated from patients is generally more than healthy carriers.
Material and Methods: Total 206 MRSA and MSSA isolates (57 MRSA and 149 MSSA isolates) obtained from 120 inpatients and 86 healthy carriers in the Gorgan were tested. Sugar fermentation carried out in phenol red broth medium, containing glucose, galactose, arabinose, fructose, xylose, rhamnose, mannose, sucrose, trehalose, raffinose or maltose . Change in color after 24 hours incubation at 37°C were recorded. The data were analyzed by SPSS software, using Chi squared test and P<0.05 was considered as meaningful.
Results: The fermentation of glucose at the 4th hour of incubation happened in 10(17.5%) and 11(7.4%) of MRSA and MSSA isolates respectively (P=0.03). The ability ferment rhamnose and xylose in MRSA isolates were 11 (19.3%) and 6 (10.5%), and in MSSA strains were 3 (2%) and 4 (2.7%) respectively. The differences between MRSA and MSSA isolates was statistically significant (P<0.05). The highest difference between S.aureus which isolated from patients and carriers were seen in fermentation of fructose [120 (100%) vs 80 (93%)], raffinose [46 (38.3%) vs 2 (2.3%)] and rhamnose [14 (11.7%) vs 0 (0%)] (P<0.005). The ability of S.aureus isolates with ability of raffinose fermentation isolated from urinary tract infections was 23 (%56.1), which is significant (P=0.03).
Conclusion: Methicillin resistant in Staphylococcus aureus isolates is accompanied with increased ability of sugars fermentation. The ability of sugars fermentation in Staphylococcus aureus isolated from patients is generally more than healthy carriers.
Type of Study: Original Research Article |
Subject:
Medical Bacteriology
Received: 2013/10/27 | Accepted: 2013/11/10 | ePublished: 2013/11/10
Received: 2013/10/27 | Accepted: 2013/11/10 | ePublished: 2013/11/10
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Copyright Policy
Iranian Journal of Medical Microbiology by Farname is licensed under CC BY-NC 4.0
