year 4, Issue 3 (Fall 2010)
Iran J Med Microbiol 2010, 4(3): 14-20 |
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Mirnejad R, Amirmozafari N, Kazemi B. Molecular identification and genotyping of Mycoplasma genitalium in women with genital infections by PCR-RFLP . Iran J Med Microbiol 2010; 4 (3) :14-20
URL: http://ijmm.ir/article-1-31-en.html
URL: http://ijmm.ir/article-1-31-en.html
1- Molecular Biology Research Center, Baqiyatallah(a.s.) University of Medical Sciences, Tehran, Iran.
2- Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
3- Cell and Molecular Biology Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran.
2- Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
3- Cell and Molecular Biology Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran.
Abstract: (17121 Views)
Background and Objectives: Mycoplasma genitalium is extremely difficult to be isolated from clinical specimens thus, it may not be possible to type them on the base on phenotype techniques. However, the availability of complete genome sequence of bacteria has offered an opportunity to approach these goals. The aim of this study was molecular identification and genotyping of Mycoplasma using PCR-RFLP in women with genital infections.
Materials & Methods: Genital swabs were taken from 210 patients, who referred to gynecology clinic of Rasool hospital between years 2007 and 2008. Samples were transported to laboratory in PBS buffer. The PCR assays were performed, using a genus specific primer pair and amplified a 465 bp fragment of 16S rRNA. Samples containing bands were subjected to digestion with restriction enzymes (AluI,Taq I, CacI8, BbsI, EcoRI).
Results: Of the 210 genital swabs tested, 11 samples (5.2%) showed positive reactions in PCR for M. genitalium, that all showed the same genotype and sequence as strain Mycoplasma genitalium G37. It was also understood that only the restriction enzyme CacI8 can cut M. genitalium genome and so it can be used as a marker to identify and type the organism.
Conclusion: This study showed that M. genitalium can be isolated from genital tracts. It also suggested that there is no heterogeneity between the isolates as genomic enzyme patterns of isolates were the same. This probably means that all infections in individuals were originated from the same ancestor.
Materials & Methods: Genital swabs were taken from 210 patients, who referred to gynecology clinic of Rasool hospital between years 2007 and 2008. Samples were transported to laboratory in PBS buffer. The PCR assays were performed, using a genus specific primer pair and amplified a 465 bp fragment of 16S rRNA. Samples containing bands were subjected to digestion with restriction enzymes (AluI,Taq I, CacI8, BbsI, EcoRI).
Results: Of the 210 genital swabs tested, 11 samples (5.2%) showed positive reactions in PCR for M. genitalium, that all showed the same genotype and sequence as strain Mycoplasma genitalium G37. It was also understood that only the restriction enzyme CacI8 can cut M. genitalium genome and so it can be used as a marker to identify and type the organism.
Conclusion: This study showed that M. genitalium can be isolated from genital tracts. It also suggested that there is no heterogeneity between the isolates as genomic enzyme patterns of isolates were the same. This probably means that all infections in individuals were originated from the same ancestor.
Type of Study: Original Research Article |
Subject:
Medical Bacteriology
Received: 2013/10/27 | Accepted: 2013/11/10 | ePublished: 2013/11/10
Received: 2013/10/27 | Accepted: 2013/11/10 | ePublished: 2013/11/10
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