year 5, Issue 3 (Fall 2011)
Iran J Med Microbiol 2011, 5(3): 29-37 |
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1- Faculty of Basic Science , Azad University
2- department of drug and food control, Pharmacy Faculty, Medical University of Tehran , fazelimoSinatums@yahoo.com
3- department of drug and food control, Pharmacy Faculty, Medical University of Tehran
4- R&D department of Zist-Daro company
2- department of drug and food control, Pharmacy Faculty, Medical University of Tehran , fazelimoSinatums@yahoo.com
3- department of drug and food control, Pharmacy Faculty, Medical University of Tehran
4- R&D department of Zist-Daro company
Abstract: (26321 Views)
Background: EMCV virus belongs to the Picornaviride family and Cardioviruses
genus. Replication results of EMCV is applicable for L929 and A549 cell lines on
studies related to measure the potency of proteins such as interferon and biological
assessment and other various researches. In order to choose the appropriate virus cell
line, comparison of the EMCV virus replication in A549 and L929 cell lines is
experimentally investigated. The aim of the present study is to compare the kinetic of
growth of EMCV in A549and L929cell lines.
Material and method: The goal of this study is to investigate the effect of the EMCV virus with the determination of the virus titer (CCID50) after passing five days on A549 and L929 cell lines under same condition. After a specific time (5 days), these cell lines are cultivated with the same virus in different densities with Reed-Munch method. MTT coloring and OD assessment are carried out with the Elayza reader system.
Result: The A549 virus titer obtained from Reed-Munch method after 24 hours reached 10 1.5 CCID50/ ml, while the virus titer of 10 3.5 CCID50/ml is obtained for L929 virus. In the following days the more virus titer is reported for L929 virus. A549 virus titer stopped after 3days with the quantity of 10 3.5 CCID50 /ml while the L929 cell line titer continued to grow for the next four days and reached 10 6.5 CCID50/ml. . MTT coloring was done after CPE study and virus titers of 10 3.5 CCID50/ml and 10 6.5 CCID50/ml are reported for the A549 and L929 respectively.
Conclusion: Although EMCV virus has an ability to grow in A549 and L929 cell lines, the growth kinetic of the L929is faster than A549. Showing the higher virus titer, it can be concluded that L929is more appropriate for the EMCV cultivation. In studies related to EMCV, Using L929cell line in studies causes less time and cost to be consumed.
Material and method: The goal of this study is to investigate the effect of the EMCV virus with the determination of the virus titer (CCID50) after passing five days on A549 and L929 cell lines under same condition. After a specific time (5 days), these cell lines are cultivated with the same virus in different densities with Reed-Munch method. MTT coloring and OD assessment are carried out with the Elayza reader system.
Result: The A549 virus titer obtained from Reed-Munch method after 24 hours reached 10 1.5 CCID50/ ml, while the virus titer of 10 3.5 CCID50/ml is obtained for L929 virus. In the following days the more virus titer is reported for L929 virus. A549 virus titer stopped after 3days with the quantity of 10 3.5 CCID50 /ml while the L929 cell line titer continued to grow for the next four days and reached 10 6.5 CCID50/ml. . MTT coloring was done after CPE study and virus titers of 10 3.5 CCID50/ml and 10 6.5 CCID50/ml are reported for the A549 and L929 respectively.
Conclusion: Although EMCV virus has an ability to grow in A549 and L929 cell lines, the growth kinetic of the L929is faster than A549. Showing the higher virus titer, it can be concluded that L929is more appropriate for the EMCV cultivation. In studies related to EMCV, Using L929cell line in studies causes less time and cost to be consumed.
Type of Study: Original Research Article |
Subject:
Medical Virology
Received: 2013/12/28 | Accepted: 2013/12/28 | ePublished: 2013/12/28
Received: 2013/12/28 | Accepted: 2013/12/28 | ePublished: 2013/12/28
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