year 3, Issue 1 (Spring 2009)                   Iran J Med Microbiol 2009, 3(1): 43-48 | Back to browse issues page

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Haghshenas, Rafiei, Zabihian, Naghshvar. Frequency of Epstein Barr Virus in esophageal squamous cell carcinoma biopsies in Mazandaran and Golestan provinces in 2008. Iran J Med Microbiol 2009; 3 (1) :43-48
URL: http://ijmm.ir/article-1-144-en.html
1- Department of Microbiology and Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran , aghshenas2001@yahoo.com
2- Molecular and Cellular Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran
3- Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
4- Department of Pathology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
Abstract:   (19220 Views)
Background and Objectives: Esophageal squamous cell carcinoma (ESCC) is one of the most common malignancies in a few region of northern Iran (Mazandaran and Golestan). Epstein Bar Virus (EBV) infection is an important cause of many human malignancies including Burkett's lymphoma and Hodgkin's disease. EBV has also been associated with nasopharyngeal and gastric cancers. The aim of this study was to determine the prevalence of EBV in biopsy sections obtained from patients with ESCC in Mazandaran and Golestan provinces in 2008.
Material and Method:This was a cross-section descriptive study on 40 surgical specimens of clinically and pathologically confirmed esophageal cancer. DNA was extracted from paraffin-embedded blocks using a commercial DNA extraction kit. EBV-DNA was amplified by using EBV-sequence specific primers using polymerase chain reaction. DNA amplification product was revealed by electrophoresis on 1% agarose gel.
Results:The biopsy sections were belonged to 22 (55%) male and 18 (45%) female patients. We found EBV in 4 (10%) of 40 specimens. Three (13.2%) of 4 EBV-positive biopsies denoted to males and the only one (5.5%) was female.
Conclusion: Identification of EBV in 10% of esophageal cancer biopsies, might suggests a role of EBV in ESCC in Northern Iran.
Keywords: ESCC, EBV, PCR
Full-Text [PDF 225 kb]   (5209 Downloads)    
Type of Study: Original Research Article | Subject: Medical Virology
Received: 2013/11/21 | Accepted: 2013/11/21 | ePublished: 2013/11/21

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