year 2, Issue 3 And 4 (3-2009)
Iran J Med Microbiol 2009, 2(3 And 4): 9-17 |
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Mobasher Kare Jeddi A, Nahaei M, Mobayyen H, Pornour M, Sadeghi J. Molecular study of extended-spectrum beta-lactamase (SHVtype) in Esherichia coliand Klebsiella pneumoniaeisolated from Medical Centers of Tabriz. Iran J Med Microbiol 2009; 2 (3 and 4) :9-17
URL: http://ijmm.ir/article-1-123-en.html
URL: http://ijmm.ir/article-1-123-en.html
Alireza Mobasher Kare Jeddi1 
, Mohammadreza Nahaei 2, Hayedeh Mobayyen3 , Majid Pornour1 , Javid Sadeghi4
, Mohammadreza Nahaei 2, Hayedeh Mobayyen3 , Majid Pornour1 , Javid Sadeghi4
1- Department of Microbiology, Zanjan Islamic Azad University, Zanjan, Iran
2- Department of Microbiology, Faculty of Medicine, Tabriz University of medical.scinces, Tabriz, Iran , nahaeimr@tbzmed.ac.ir
3- Drug Applied Research Center, Tabriz University of medical.scinces, Tabriz, Iran
4- Department of Microbiology, Faculty of Medicine, Tabriz University of medical.scinces, Tabriz, Iran
2- Department of Microbiology, Faculty of Medicine, Tabriz University of medical.scinces, Tabriz, Iran , nahaeimr@tbzmed.ac.ir
3- Drug Applied Research Center, Tabriz University of medical.scinces, Tabriz, Iran
4- Department of Microbiology, Faculty of Medicine, Tabriz University of medical.scinces, Tabriz, Iran
Abstract: (17407 Views)
Background and objectives: Nowadays, there is an emerging increase in drug resistance in medical centers due
to extended use of third generation cephalosporins. This study was conducted to evaluate antibiotic sensitivity
profiles of Esherichia coli and Klebsiella pneumoniae isolates collected from four medical centers (Imam
Khomeini, Sina, Shohada and Pediatrics) in Tabriz and detection of presence of blaSHV gene among the isolates.
Materials and methods: In total 41 isolates of Escherichia coli and 47 isolates of Klebseilla pneumoniae were collected from medical centers. The antibiotic sensitivity pattern of the isolated were determined by using drug susceptibility test according to Kirby-Bauer method. Combined disk test was also used as confirmatory test, and the findings were adapted with CLSI standard. PCR technique was used for investigation of presence blaSHV gene in final step .
Results: Among the E. coli and K. pneumoniae isolates, 33 (80.49%) and 43 (91.48%) were resistant to ceftazidime and 32(78.05%) and 42 (89.36%) were resistant to cefotaxime respectively. Forthy out of 41 E. coli isolates (97.5%) were ESBL producers, which among them 7 isolates (17.0%) contained blaSHV gene. Among 47 K. pneumoniae isolates, 46 (97.8%) were ESBL producers which 12 isolates (25.5%) contained blaSHV gene. All of the E. coli and K. pneumoniae isolates were sensitive to imipenem.
Conclusion: The high resistance profiles encountered in E. coli and K. pneumoniae isolates, underlines need for accurate sensitivity testing and also avoidance from inappropriate use of antibiotics in management of ESBL producing bacteria is recommended.
Materials and methods: In total 41 isolates of Escherichia coli and 47 isolates of Klebseilla pneumoniae were collected from medical centers. The antibiotic sensitivity pattern of the isolated were determined by using drug susceptibility test according to Kirby-Bauer method. Combined disk test was also used as confirmatory test, and the findings were adapted with CLSI standard. PCR technique was used for investigation of presence blaSHV gene in final step .
Results: Among the E. coli and K. pneumoniae isolates, 33 (80.49%) and 43 (91.48%) were resistant to ceftazidime and 32(78.05%) and 42 (89.36%) were resistant to cefotaxime respectively. Forthy out of 41 E. coli isolates (97.5%) were ESBL producers, which among them 7 isolates (17.0%) contained blaSHV gene. Among 47 K. pneumoniae isolates, 46 (97.8%) were ESBL producers which 12 isolates (25.5%) contained blaSHV gene. All of the E. coli and K. pneumoniae isolates were sensitive to imipenem.
Conclusion: The high resistance profiles encountered in E. coli and K. pneumoniae isolates, underlines need for accurate sensitivity testing and also avoidance from inappropriate use of antibiotics in management of ESBL producing bacteria is recommended.
Keywords: Extended-spectrum beta-lactamase, Escherichia coli, K. pneumoniae, Polymrase chain reaction, blaSHVgene.
Type of Study: Original Research Article |
Subject:
Molecular Microbiology
Received: 2013/11/15 | Accepted: 2013/11/16 | ePublished: 2013/11/16
Received: 2013/11/15 | Accepted: 2013/11/16 | ePublished: 2013/11/16
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