1- Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran
2- Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran , mpourmand@tums.ac.ir
Abstract: (31365 Views)
Background & Objectives: Natural staphylococcal infections and vaccines based whole bacteria lead to
poor antibody responses, but recent research reveals that specific antibodies based on recombinant
staphylococcal antigens are much more protective. Sacol is a novel antigen that its structural and
immunological traits poorly characterized. This research aimed to clone of sacol, a novel gene from
Staphylococcus aureus.
Material and Methods: The specific primers with suitable restriction sites were designed and sacol
amplified by PCR. The sacoland plasmid were produced as sticky ends by restriction enzymes NdeI and
XhoI. To amplify the recombinant plasmid the pET21sacol transferred into competent cell E.coli TOP10. The
recombinant plasmid harvested from the host and analyzed by restriction enzymes and sequencing. Finally,
sacolgene analyzed by bioinformatics tools.
Results: The sacolgene has 723bp which amplified, cloned and sequenced successfully. Sacol is highly
conserved in Staphylococcus aureus strains. Moreover, software analysis shows that sacolencodes a protein
with 32KDa molecular weight (267 amino acids) which has similarity with C51 peptidase in N-terminal with
one alpha helix and 14 beta sheets.
Conclusion: the sacolgene is conserved in majority of Staphylococcus aureus strains and may exist and
express in most of staphylococcal infections.The role and regulation of the gene is thus of great interest.
Type of Study:
Original Research Article |
Subject:
Microbial Biotechnology Received: 2013/11/14 | Accepted: 2013/11/14 | ePublished: 2013/11/14