Mirzaee M, Gharib A, Owlia P. Comparison of antimicrobial effect offree and liposomal amikacin against Pseudomonas aeruginosa. Iran J Med Microbiol 2008; 2 (2) :43-48
URL:
http://ijmm.ir/article-1-119-en.html
1- Department of Lab. Sciences, Faculty of Medicine, Azad University, Borujerd, Iran
2- Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran. , owlia@shahed.ac.ir
Abstract: (23771 Views)
Background and objectives: Pseudomonas aeruginosa is important opportunistic pathogen and to produce
widespread infection by numerous virulence factors. Drug delivery system that reduces the drugs toxicity
while increasing their therapeutic index is a great interest and liposomes can provide the benefits. Liposomes
are colloidal vesicules ranging from a few nanometers to several micrometers in diameter. The present in
vitro study was designated to evaluate the antimicrobial activity of free and liposomal amikacin against
Pseudomonas aeruginosa(ATCC 27853).
Materials and methods: The minimal inhibitory concentrations (MICs) of free and liposomal amikacin for
Pseudomonas aeruginosa (ATCC 27853) were determined by broth macro-dilution technique as
recommended by CLSI (Clinical and laboratory standards institute). We therefore, encapsulated this drug in
to liposome prepared by sonication. Change of number of bacteria in equal minimal inhibitory concentration
of liposomal amikacin was compared with the change of number of bacteria in the present of various
concentrations of free amikacin.
Results:The results showed that of liposomal amikacinhad antimicrobial effect and MIC of liposomal
amikacin were equal of 4µg/ml however minimal inhibitory concentration of free amikacin was 2µg/ml.
Comparison of change of number of bacteria had shown that the effect of liposomal amikacin (4 µg/ml) after
8 h is equal of 6 µg/ml free amikacin after 4h.
Conclusion: According to the results we can probably to use of liposomal amikacin for Pseudomonas
infections, but we need more study and research
Type of Study:
Original Research Article |
Subject:
Antimicrobial Substances Received: 2013/11/15 | Accepted: 2013/11/15 | ePublished: 2013/11/15