year 2, Issue 2 (Summer 2008)                   Iran J Med Microbiol 2008, 2(2): 43-48 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mirzaee M, Gharib A, Owlia P. Comparison of antimicrobial effect offree and liposomal amikacin against Pseudomonas aeruginosa. Iran J Med Microbiol 2008; 2 (2) :43-48
URL: http://ijmm.ir/article-1-119-en.html
1- Department of Lab. Sciences, Faculty of Medicine, Azad University, Borujerd, Iran
2- Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran. , owlia@shahed.ac.ir
Abstract:   (23771 Views)
Background and objectives: Pseudomonas aeruginosa is important opportunistic pathogen and to produce widespread infection by numerous virulence factors. Drug delivery system that reduces the drugs toxicity while increasing their therapeutic index is a great interest and liposomes can provide the benefits. Liposomes are colloidal vesicules ranging from a few nanometers to several micrometers in diameter. The present in vitro study was designated to evaluate the antimicrobial activity of free and liposomal amikacin against Pseudomonas aeruginosa(ATCC 27853).
Materials and methods: The minimal inhibitory concentrations (MICs) of free and liposomal amikacin for Pseudomonas aeruginosa (ATCC 27853) were determined by broth macro-dilution technique as recommended by CLSI (Clinical and laboratory standards institute). We therefore, encapsulated this drug in to liposome prepared by sonication. Change of number of bacteria in equal minimal inhibitory concentration of liposomal amikacin was compared with the change of number of bacteria in the present of various concentrations of free amikacin.
Results:The results showed that of liposomal amikacinhad antimicrobial effect and MIC of liposomal amikacin were equal of 4µg/ml however minimal inhibitory concentration of free amikacin was 2µg/ml. Comparison of change of number of bacteria had shown that the effect of liposomal amikacin (4 µg/ml) after 8 h is equal of 6 µg/ml free amikacin after 4h.
Conclusion: According to the results we can probably to use of liposomal amikacin for Pseudomonas infections, but we need more study and research
Full-Text [PDF 328 kb]   (3675 Downloads)    
Type of Study: Original Research Article | Subject: Antimicrobial Substances
Received: 2013/11/15 | Accepted: 2013/11/15 | ePublished: 2013/11/15

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Journal of Medical Microbiology

Designed & Developed by : Yektaweb | Publisher: Farname Inc